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Biology A (Gateway) · OCR J247 · Paper 4 · Higher · June 2025

OCR GCSE Biology A (Gateway) Paper 4 Higher June 2025 question paper J247/04

1h 45m 90 marks

OCR J247 · Paper 4

23 questions
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Question 1

A student is investigating variation by collecting data on shoe sizes. These are the shoe sizes of six members of their family.

Figure for question 1

Which of these statements about the data is correct ?

A The mean shoe size and the modal shoe size are the same.

B The mean shoe size is 6.

C The mean shoe size is larger than the modal shoe size.

D The modal shoe size is 7.

Your answer

[1]

Answer
Mark scheme for question 1

Question 2

Figure for question 2

[1]

Answer
Mark scheme for question 2

Question 3

What is an adaptation of white blood cells?

A They can change shape to squeeze out of blood vessels into tissues.

B They have many ribosomes to produce antibiotics.

C They lack a nucleus so that they can engulf bacteria.

D They produce enzymes to make blood clot.

Your answer

[1]

Answer
Mark scheme for question 3

Question 4

What is the correct definition of hydroponics?

A A process for growing plants in a mineral solution

B A process in the water cycle

C The movement of sweat out of sweat glands in the skin

D The movement of water in xylem vessels

Your answer

[1]

Answer
Mark scheme for question 4

Question 5

An organism has the alleles Aa for a gene.

What do the Aa alleles represent?

A A heterozygous genotype

B A heterozygous phenotype

C A homozygous genotype

D A homozygous phenotype

Your answer

[1]

Answer
Mark scheme for question 5

Question 6

Which of these units of genetic material is largest ?

A Base

B Chromosome

C Gene

D Genome

Your answer

[1]

Answer
Mark scheme for question 6

Question 7

The flow chart shows some developments in our understanding of genetics.

Figure for question 7

Which stage on the flow chart represents the ideas of Mendel?

Your answer

[1]

Answer
Mark scheme for question 7

Question 8

Which of these diseases is caused by a bacterium?

A AIDS

B Barley powdery mildew

C Crown gall disease

D Tobacco mosaic disease

Your answer

[1]

Answer
Mark scheme for question 8

Question 9

A muscle cell in a horse has 64 chromosomes.

Which of these statements is correct ?

A A horse egg cell has 64 chromosomes and is haploid.

B A horse liver cell has 32 chromosomes and is haploid.

C A horse neurone has 64 chromosomes and is diploid.

D A horse sperm cell has 32 chromosomes and is diploid.

Your answer

[1]

Answer
Mark scheme for question 9

Question 10

The diagram shows how carbon is recycled in nature.

Figure for question 10

Which of the processes 1, 2, 3 and 4 may require oxygen?

A 1, 2, 3 and 4

B 1, 2 and 4 only

C 1 and 4 only

D 1 only

Your answer

[1]

Answer
Mark scheme for question 10

Question 11

Which description is correct for human sperm cells?

A They always contain an X chromosome.

B They always contain a Y chromosome.

C They contain an X and a Y chromosome.

D They contain either an X or a Y chromosome.

Your answer

[1]

Answer
Mark scheme for question 11

Question 12

The diagram shows the flow of biomass through a food chain.

Figure for question 12

What is the combined biomass of all the predators in this food chain?

400 kg / km2 A

2400 kg / km2 B

22400 kg / km2 C

20 022 400 kg / km2 D

Your answer

[1]

Answer
Mark scheme for question 12

Question 13

Clinical testing is used for new medicines.

What is a feature of clinical testing?

A Uses animals

B Uses cell cultures

C Uses computer models

D Uses placebos

Your answer

[1]

Answer
Mark scheme for question 13

Question 14

What is not a proven use for monoclonal antibodies?

A Gene therapy

B Pregnancy tests

C Targeting cancer cells

D Testing for cancer

Your answer

[1]

Answer
Mark scheme for question 14

Question 15

How may a mutation in non-coding DNA result in a change in phenotype?

A It changes the amino acid sequence of a protein.

B It changes the shape of the active site of a protein.

C It prevents mRNA binding with ribosomes.

D It prevents transcription occurring.

Your answer

[1]

Answer
Mark scheme for question 15

Question 16

Many different bacteria can cause disease.

(a)

State the name given to any organism that causes disease.

[1]

Answer
Mark scheme for question 16(a)
(b)

A patient in hospital has a bacterial disease. A doctor tells the patient:

‘Some time ago, bacteria entered your body.

Your body detected chemicals on the surface of the bacteria.

This made your body produce protein molecules to help kill the bacteria.

I will give you a tablet containing a medicine that will also kill the bacteria.

You should not get this disease again because cells in your body will now provide you with immunity.’

(b)(i)

The boxes contain descriptions used by the doctor and some biological terms.

Draw four lines to connect each description to its correct biological term.

Figure for question 16

[4]

Answer
Mark scheme for question 16(b)(i)
(b)(ii)

The doctor would change the type of medicine given to the patient if the disease was caused by a virus.

State the change the doctor would make.

Give a reason for your answer.

Change........

Reason........

[2]

Answer
Mark scheme for question 16(b)(ii)

Question 17

Figure for question 17
(a)

The students sample the pitch to find the mean number of dandelion plants per m2 in each part. (a)

Explain how the students use a quadrat to find the mean number of dandelion plants per m2 in a part of the pitch.

[3]

Answer
Mark scheme for question 17(a)
(b)
Figure for question 17

[3]

Answer
Mark scheme for question 17(b)
(c)

The students write a conclusion for their experiment.

Complete the students’ conclusion.

Use words from the list.

community compete differently efficiently equally

habitat mutate population respire

The football pitch contains a........ of different plant species, mainly grass and dandelions.

The part of the pitch that has the most players running on it has the most dandelions.

This is because it is more difficult for plants to grow there.

The dandelions successfully........ with the grass in these areas.

We can only make this conclusion if abiotic factors affect each area........

[3]

Answer
Mark scheme for question 17(c)
(d)

Describe how the pupils could extend their experiment to show that soil pH was the same in each part of the pitch.

[2]

Answer
Mark scheme for question 17(d)

Question 18

Locusts are insects that live in many parts of Africa.

They eat many different types of plants.

Figure for question 18

One female locust can lay 50 eggs and they take 10 days to hatch.

A large group of locusts is called a swarm and contains one thousand million locusts.

A swarm can fly 80 km in a day.

(a)

Give the number of locusts in a swarm in standard form.

[1]

Answer
Mark scheme for question 18(a)
(b)

Some students want to find out how long it would take a swarm of locusts to eat all the grass in a field.

This is the method they use:

• Keep one locust in a cage in their laboratory.

• Give the locust grass to eat.

• Measure the mass of grass that the locust eats in one hour.

• Find the mass of grass in the whole field.

These are the students’ results:

Mass of grass eaten by one locust in one hour = 0.001 kg Mass of grass in a field = 5 000 kg

(b)(i)

Calculate how long it would take for a swarm of locusts to eat all the grass in the field.

[3]

Answer
Mark scheme for question 18(b)(i)
(b)(ii)

Suggest one way that the students could improve their method to get a more accurate estimate of the time.

[1]

Answer
Mark scheme for question 18(b)(ii)
(c)

Explain why locusts are a major risk to food security in Africa.

Use information from all of question 18.

[3]

Answer
Mark scheme for question 18(c)

Question 19

SIOD is an inherited genetic disorder.

The diagram shows a family tree with a person who has SIOD.

Figure for question 19
(a)

SIOD is caused by a recessive allele.

Explain how you can tell from the family tree that the allele for SIOD is recessive.

[2]

Answer
Mark scheme for question 19(a)
(b)
Figure for question 19

[3]

Answer
Mark scheme for question 19(b)
(c)

One of the symptoms of SIOD is kidney failure.

People with the disorder often need a kidney transplant.

(c)(i)

After receiving a kidney transplant from a donor, a person needs to take drugs to reduce the activity of their immune system.

Explain why it is necessary to reduce the activity of their immune system after a transplant.

[2]

Answer
Mark scheme for question 19(c)(i)
(c)(ii)

Give a possible disadvantage of having to take drugs to reduce the activity of the immune system.

[1]

Answer
Mark scheme for question 19(c)(ii)
(c)(iii)

The first person to receive stem cell treatment for SIOD was in 2023.

This person received stem cells from a donor.

These stem cells reached the bone marrow and produced large numbers of white blood cells.

Explain why stem cells can produce large numbers of white blood cells.

[2]

Answer
Mark scheme for question 19(c)(iii)
(c)(iv)

Six months later, the person received a kidney transplant from the same donor.

Why would this person not need to take drugs to reduce the activity of their immune system?

[1]

Answer
Mark scheme for question 19(c)(iv)

Question 20

The diagram shows a food web from a lake in North America.

Figure for question 20
(a)

Explain why carp are described as feeding at two different trophic levels.

Use details from the food web.

[2]

Answer
Mark scheme for question 20(a)
(b)

Carp are large fish that have been introduced into America from Asia.

Their numbers in lakes have increased and they are becoming pests.

Suggest a reason why the population of a species often increases rapidly after they are introduced into a new area.

[1]

Answer
Mark scheme for question 20(b)
(c)

Carp feed at the bottom of lakes and this stirs up mud into the water.

The mud contains minerals but makes the water cloudy.

Explain two different effects that this could have on the microscopic plants living in the lake.

[4]

Answer
Mark scheme for question 20(c)
(d)

* Carp embryos develop into male or female fish depending on the level of female hormone they produce.

If carp embryos produce high levels of the female hormone, oestrogen, they will become females.

Scientists are trying to reduce the numbers of carp in lakes by using genetic engineering.

This is their plan:

• introduce a gene into some early carp embryos that will stop the production of oestrogen

• release the genetically modified carp into the lakes.

Describe how the gene could be introduced into carp cells and explain how the scientists’ plan could decrease the number of carp in the lake.

[6]

Answer
Mark scheme for question 20(d)

Question 21

People can have their DNA tested.

The table shows five different types of DNA test and the purpose of each test.

Figure for question 21
(a)

Which type of test, A, B, C, D or E, is identifying risk factors?

[1]

Answer
Mark scheme for question 21(a)
(b)

Which type of test, A, B, C, D or E, will not affect the person being tested but might be useful if they are planning to have children?

[1]

Answer
Mark scheme for question 21(b)
(c)

Test E can test for a cancerous tumour.

Describe how cancerous tumours form.

[2]

Answer
Mark scheme for question 21(c)
(d)

Deciding whether to take test C can be a difficult decision for some people.

Suggest one possible reason why.

[1]

Answer
Mark scheme for question 21(d)

Question 22

Farmers sometimes give their farm animals antibiotics even if the animals only have harmless bacteria living in their body.

This is to increase the growth rate of the animals.

(a)

Suggest why harmless bacteria living in the digestive system of animals could decrease the growth rate of the animals.

[1]

Answer
Mark scheme for question 22(a)
(b)

Scientists have been studying the bacteria found in different farm animals.

They took samples from different farm animals and tested them to see if bacteria in the samples were killed by different antibiotics.

(b)(i)

Describe how bacteria can be tested in the laboratory to see if they are killed by an antibiotic.

[3]

Answer
Mark scheme for question 22(b)(i)
(b)(ii)
Figure for question 22

[3]

Answer
Mark scheme for question 22(b)(ii)
(c)

A student writes three sentences about how antibiotic resistance might occur:

1. ‘Bacteria mutate when the DNA in their nucleus changes.’

2. ‘Antibiotics cause these mutations to occur.’

3. ‘These mutations can produce an allele that makes the bacteria immune to antibiotics.’

Identify the mistake the student has made in each sentence.

[3]

Answer
Mark scheme for question 22(c)

Question 23

A bacterium causes a disease in cucumber plants called bacterial wilt.

Here are some details of the disease:

• Bacteria causing the disease are found on beetles.

• The beetles feed on cucumber plants.

• Inside the plant, the bacteria produce a sticky substance in the xylem vessels.

• The leaves of the plants then wilt and die.

(a)

Explain how a beetle feeding on a cucumber plant causes the leaves of the plant to wilt from this disease.

[4]

Answer
Mark scheme for question 23(a)
(b)

Spraying the surface of the plant with chemicals that kill bacteria does not prevent the disease spreading.

Suggest a reason why.

[1]

Answer
Mark scheme for question 23(b)
(c)

Scientists are trying to find a different way of killing the bacteria.

They plan to use another organism to kill the bacteria.

(c)(i)

What is the name of this type of control method?

[1]

Answer
Mark scheme for question 23(c)(i)
(c)(ii)

The organism that the scientists plan to use is a virus.

The virus produces an enzyme that breaks down the bacterial cell wall.

Explain why this may cause the bacteria to swell up and burst.

[2]

Answer
Mark scheme for question 23(c)(ii)
(c)(iii)

The scientists added the virus to the bacteria and measured how effective it was at killing the bacteria. They did this at different temperatures.

Their results are shown in the graph.

Figure for question 23

The scientists concluded that the virus could be used to treat bacterial wilt throughout the year.

Use the graph to explain why the scientists made this conclusion.

[2]

Answer
Mark scheme for question 23(c)(iii)

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