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Edexcel GCSE Combined Science

Topic questions Ecology The nitrogen cycle

The nitrogen cycle

8 questions · 85 marks · about 1.5 hours

The nitrogen cycle questions

Edexcel 1SC0 The nitrogen cycle 8 questions

Paper 2: Biology 2 June 2025 Foundation tier, Question 3 10 marks

Figure 3 shows part of the nitrogen cycle.

Figure for question 3
(a)

Use words from the box to complete the following sentences about the nitrogen cycle.

Figure for question 3

The nitrogen compounds in plants include........

The nitrates from rotting dead plants are released into the soil by........

[2]
Reveal mark scheme Mark scheme for question 3(a)
(b)

180 million tonnes of nitrates were used by crops growing on farms in the UK in 2020.

Figure 4 shows the different sources of nitrates used by the crops.

Figure for question 3
(b)(i)

Calculate the mass of nitrates from human-made fertiliser that was used by crops in 2020.

[2]
Reveal mark scheme Mark scheme for question 3(b)(i)
(b)(ii)

Some of the nitrates spread on fields as fertiliser get into lakes.

Name the type of pollution caused by the build-up of nitrates in lakes.

[1]
Reveal mark scheme Mark scheme for question 3(b)(ii)
(c)

A student investigated how the concentration of nitrate affected the types of algae growing in three lakes.

Figure 5 shows microscope images of the algae in 1 mm3 of water, and the concentrations of nitrate ions in each lake.

Figure for question 3
(c)(i)

Figure 6 shows a bar chart for the algae in lakes X, Y and Z.

Complete the bar chart for algae L and algae M in lake Z.

Figure for question 3
[2]
Reveal mark scheme Mark scheme for question 3(c)(i)
(c)(ii)

State two conclusions about the relationship between nitrate concentration and the algae in these lakes.

[2]
Reveal mark scheme Mark scheme for question 3(c)(ii)
(c)(iii)

A build-up of algae on the surface of the lake can affect plants growing at the bottom of the lake.

Which statement shows how this would affect the plants at the bottom of the lake?

Question 3, shown as an image
[1]
Reveal mark scheme Mark scheme for question 3(c)(iii)

Paper 2: Biology 2 June 2024 Higher tier, Question 3 10 marks

(a)

A scientist decided to study the variety of living organisms in a garden.

(a)(i)

The scientist wanted to use a random sampling technique.

Devise a plan the scientist could use to randomly sample the number of plant species in the garden.

[3]
Reveal mark scheme Mark scheme for question 3(a)(i)
(a)(ii)

The scientist also measured abiotic factors in the garden.

The pH of the soil was measured using a pH meter.

Describe how three other abiotic factors could be measured in the garden.

[3]
Reveal mark scheme Mark scheme for question 3(a)(ii)
(a)(iii)

Mistletoe is a parasite that grows on some trees.

Figure 4 shows a tree with mistletoe growing on it.

Figure for question 3

Describe the feeding relationship between the mistletoe and the tree.

[2]
Reveal mark scheme Mark scheme for question 3(a)(iii)
(b)

Nitrate fertilisers are used in the garden.

Explain why nitrate fertilisers are used in gardens.

[2]
Reveal mark scheme Mark scheme for question 3(b)

Paper 2: Biology 2 June 2023 Higher tier, Question 6 12 marks

Figure 9 shows a photomicrograph of two stomata in a leaf.

Figure for question 6
(a)
(a)(i)

The length of one guard cell in this image is 6 mm.

Convert the length of this guard cell into micrometres (µ m).

[1]
Reveal mark scheme Mark scheme for question 6(a)(i)
(a)(ii)

The image has been magnified 150×.

Calculate the actual size of the guard cell.

Give your answer in standard form in mm.

........ mm

[3]
Reveal mark scheme Mark scheme for question 6(a)(ii)
(b)

Explain the role of denitrifying bacteria in the nitrogen cycle.

[2]
Reveal mark scheme Mark scheme for question 6(b)
(c)

*(c) Describe how carbon is cycled through the biotic and abiotic components of an ecosystem.

[6]
Reveal mark scheme Mark scheme for question 6(c)

Paper 2: Biology 2 June 2022 Higher tier, Question 3 9 marks

(a)

Figure 4 shows a cross-section of a root nodule on a leguminous plant.

Bacteria in the root nodule provide the leguminous plant with nitrogen compounds.

The leguminous plant provides the bacteria with sugars.

Figure for question 3

(Source: © Nigel Downer / Science Photo Library)

Figure 4

(a)(i)

Which term describes the relationship between this leguminous plant and the bacteria?

Question 3, shown as an image
[1]
Reveal mark scheme Mark scheme for question 3(a)(i)
(a)(ii)

The width of this root nodule is 7.5 mm.

Give the width in µ m.

[1]
Reveal mark scheme Mark scheme for question 3(a)(ii)
(b)

Figure 5 shows part of the nitrogen cycle.

Question 3, shown as an image
Question 3, shown as an image
(b)(i)

Identify the types of microorganism involved in process X and process Y.

[2]
Reveal mark scheme Mark scheme for question 3(b)(i)
(b)(ii)

Explain how crop rotation increases nitrate levels in the soil.

[3]
Reveal mark scheme Mark scheme for question 3(b)(ii)
(b)(iii)

Explain why increased nitrate levels in the soil improve crop yield.

[2]
Reveal mark scheme Mark scheme for question 3(b)(iii)

Paper 2: Biology 2 June 2019 Foundation tier, Question 5 12 marks

(a) A student was investigating the populations of organisms in a garden.

Figure 9 shows the estimates of the number and biomass of some of the organisms in the garden.

organismnumbermean biomass of each organism in gramsbiomass of population in grams
cabbages (plants)80705600
earthworms6203.4?
slugs304.1123
hedgehogs1620620
squirrels26001200
Question 5, shown as an image
(a)
(a)(i)

Calculate the biomass of the population of earthworms in the garden.

[1]
Reveal mark scheme Mark scheme for question 5(a)(i)
(a)(ii)

Hedgehogs eat slugs and earthworms. Slug pellets were used to kill the slugs.

Explain how killing the slugs would affect the population of earthworms in this garden.

[2]
Reveal mark scheme Mark scheme for question 5(a)(ii)
(a)(iii)

Describe a method that could be used to estimate the population of slugs in the garden.

[3]
Reveal mark scheme Mark scheme for question 5(a)(iii)
(b)

Explain how cabbages, earthworms and squirrels contribute to the carbon cycle.

[3]
Reveal mark scheme Mark scheme for question 5(b)
(c)

State three ways the concentration of nitrates in soil can be increased.

[3]
Reveal mark scheme Mark scheme for question 5(c)

Paper 2: Biology 2 June 2019 Higher tier, Question 6 11 marks

(a)

Figure 6 shows the global movement of carbon into or out of the atmosphere.

Figure for question 6

Calculate the net mass of carbon added to the atmosphere each year.

........ gigatonnes

[2]
Reveal mark scheme Mark scheme for question 6(a)
(b)

Joseph Priestley was a scientist who investigated how green plants and combustion affected the carbon cycle.

Figure 7 shows his first experiment.

Figure for question 6
(b)(i)

State why the candle was not burning after three minutes.

[1]
Reveal mark scheme Mark scheme for question 6(b)(i)
(b)(ii)

Joseph Priestley continued the investigation but placed a plant inside the bell jar as shown in Figure 8.

Figure for question 6

He observed that the candle stayed alight for two minutes more than the candle in the bell jar in the first experiment.

Explain his observation.

[2]
Reveal mark scheme Mark scheme for question 6(b)(ii)
(b)(iii)

State two variables that would need to be controlled to compare these two experiments.

[2]
Reveal mark scheme Mark scheme for question 6(b)(iii)
(c)

Nitrogen is cycled through the environment.

Describe the roles of bacteria in the nitrogen cycle.

[4]
Reveal mark scheme Mark scheme for question 6(c)

Paper 2: Biology 2 June 2018 Foundation tier, Question 3 9 marks

(a) Plants use nitrate ions to make proteins and chlorophyll.

(a)
(a)(i)

What effects will a low nitrate ion concentration in soils have on plants?

Question 3, shown as an image
[1]
Reveal mark scheme Mark scheme for question 3(a)(i)
(a)(ii)

Which organisms convert nitrogen to nitrate ions during the nitrogen cycle?

Question 3, shown as an image
[1]
Reveal mark scheme Mark scheme for question 3(a)(ii)
(b)

Figure 5 shows part of a root as seen using a light microscope.

Figure for question 3

Figure 6 shows information about the two types of cell labelled in Figure 5.

Figure for question 3
(b)(i)

Calculate the surface area to volume ratio of the root hair cell.

[2]
Reveal mark scheme Mark scheme for question 3(b)(i)
(b)(ii)

Explain the benefit to the plant of having root hair cells.

[2]
Reveal mark scheme Mark scheme for question 3(b)(ii)
(c)

Algae are green plants.

Figure 7 shows the number of algae in a lake in the United Kingdom during one year.

Figure for question 3

Explain the changes in the number of algae in the lake from February to June.

[3]
Reveal mark scheme Mark scheme for question 3(c)

Paper 2: Biology 2 June 2018 Higher tier, Question 4 12 marks

(a) A student investigated the effect of nitrate ion concentration on plant growth. She placed barley seedlings in three test tubes containing different concentrations of nitrate fertiliser.

Test tube 1 contained distilled water with 1 pellet of nitrate fertiliser. Test tube 2 contained distilled water with 2 pellets of nitrate fertiliser. Test tube 3 contained distilled water with 3 pellets of nitrate fertiliser.

After 7 days, the lengths of the seedlings were measured.

Figure 6 shows an example of the apparatus used.

Figure for question 4
(a)
(a)(i)

Describe a control for this investigation.

[2]
Reveal mark scheme Mark scheme for question 4(a)(i)
(a)(ii)

The nitrate fertiliser contains the chemical compound potassium nitrate. The hazard symbol on the bag of potassium nitrate fertiliser is shown in Figure 7.

Figure for question 4

Which hazard does this symbol represent?

Question 4, shown as an image
[1]
Reveal mark scheme Mark scheme for question 4(a)(ii)
(a)(iii)

Give a method, other than measuring the change in length, that would show the growth of the seedlings.

[1]
Reveal mark scheme Mark scheme for question 4(a)(iii)
(b)

Figure 8 shows the results of this investigation.

Figure for question 4
(b)(i)

Explain why there are differences in the change in the lengths of the seedlings.

[2]
Reveal mark scheme Mark scheme for question 4(b)(i)
(b)(ii)

Explain how nitrate ions were absorbed by the seedling in test tube 3.

[3]
Reveal mark scheme Mark scheme for question 4(b)(ii)
(c)

Farmers use crop rotation to reduce the need to add nitrate fertilisers to the soil.

Plants such as peas and beans have a mutualistic relationship with nitrogen-fixing bacteria.

Explain why farmers use these plants in their crop rotation cycle.

[3]
Reveal mark scheme Mark scheme for question 4(c)

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