Question 1
Enzymes are large molecules and biological catalysts.
A catalyst changes the rate of a chemical reaction.
What happens to the mass of a catalyst during a chemical reaction?
Tick (✓) one box.
The mass of the catalyst decreases.
The mass of the catalyst stays the same.
The mass of the catalyst increases.
[1 mark]
Figure 1 shows a model of enzyme action.
Name X and Y in Figure 1.
[2 marks]
Amylase is an enzyme that breaks down starch.
A student investigated how changing the pH affected the activity of amylase.
This is the method used.
1. Heat a test tube containing 2 cm3 of amylase solution at pH 5.0 in a water bath at 35 °C.
2. Heat a test tube containing 2 cm3 of starch solution in the same water bath.
3. Transfer the amylase solution into the test tube of starch solution and mix.
4. After 30 seconds remove a drop of the amylase–starch mixture and test the drop for starch.
5. Repeat step 4 until no starch is detected.
6. Record the total time taken for no starch to be detected.
7. Repeat steps 1 to 6 using amylase solution at different pH values.
Explain why the solutions are all placed in the same water bath.
[2 marks]
Describe the test for starch.
Give the result of the test if starch is present.
[2 marks]
Table 1 shows the results.
Which range of pH values had the fastest rate of reaction?
Tick (✓) one box.
pH 5.0 to pH 5.5
pH 6.0 to pH 6.5
pH 7.0 to pH 7.5
pH 8.0 to pH 8.5
[1 mark]
How could the method be improved to obtain a more accurate pH value for the fastest rate of reaction?
[1 mark]