BREAKING
Mitochondria finally takes a day off, cell in chaos★Student draws plant cell, forgets the cell wall, again★Required practical goes wrong, cress wins★
  1. Home
  2. GCSE
  3. Biology
  4. AQA
  5. Limiting factors in photosynthesis

AQA GCSE Biology

Topic questions Plant biology Limiting factors in photosynthesis

Limiting factors in photosynthesis

12 questions · 163 marks · about 3 hours

Limiting factors in photosynthesis questions

AQA 8461 Limiting factors in photosynthesis 12 questions

Paper 1 June 2025 Foundation tier, Question 2 12 marks

A student investigated the effect of temperature on the rate of photosynthesis in pondweed. Figure 2 shows how the student set up the apparatus.

Figure for question 2

This is the method used.

1. Prepare six tubes of pondweed as shown in Figure 2.

2. Place one tube into each of six water baths set at different temperatures.

3. Wait two minutes.

4. Measure the time taken for each piece of pondweed to release 10 bubbles.

2.1

What is the independent variable in the investigation?

Tick () one box.

Figure for question 2
[1]
Reveal mark scheme Mark scheme for question 2.1
2.2

Table 2 shows the results.

Figure for question 2

Complete the sentences.

Choose answers from the box.

Figure for question 2
Question 2, shown as an image
Question 2, shown as an image
[2]
Reveal mark scheme Mark scheme for question 2.2
2.3

Describe the effect of increasing temperature on the time taken for the pondweed to release 10 bubbles.

[2]
Reveal mark scheme Mark scheme for question 2.3
2.4

Calculate the rate of photosynthesis at 50 °C.

Use the equation:

Question 2, shown as an image

Use Table 2.

Give your answer to 1 decimal place.

Question 2, shown as an image
[3]
Reveal mark scheme Mark scheme for question 2.4
2.5

Which temperature had the fastest rate of photosynthesis?

Tick () one box.

Figure for question 2
[1]
Reveal mark scheme Mark scheme for question 2.5
2.6

Table 2 is repeated below.

Figure for question 2

The student repeated the investigation at 80 °C.

What would be the time taken for the pondweed to release 10 bubbles at 80 °C?

Tick () one box.

Figure for question 2
[1]
Reveal mark scheme Mark scheme for question 2.6
2.7

Which two factors could affect the rate of photosynthesis in this investigation?

Tick () two boxes.

Figure for question 2
[2]
Reveal mark scheme Mark scheme for question 2.7

Paper 1 June 2025 Higher tier, Question 4 16 marks

A student investigated the effect of temperature on the rate of photosynthesis in pondweed.

Figure 4 shows how the student set up the apparatus.

Figure for question 4

This is the method used.

1. Prepare five tubes of pondweed as shown in Figure 4.

2. Place one tube into each of five water baths set at different temperatures.

3. Wait two minutes.

4. Measure the time taken for each piece of pondweed to release 10 bubbles.

4.1

What is the independent variable in the investigation?

[1]
Reveal mark scheme Mark scheme for question 4.1
4.2

Table 4 shows the results.

Figure for question 4

Calculate the rate of photosynthesis at 20 °C.

Give your answer to 3 significant figures.

Give the unit.

Question 4, shown as an image
[4]
Reveal mark scheme Mark scheme for question 4.2
4.3

Explain the difference in the rate of photosynthesis at 40 °C compared with the rate of photosynthesis at 10 °C.

[3]
Reveal mark scheme Mark scheme for question 4.3
4.4

Give two other factors that affect the rate of photosynthesis.

Do not refer to temperature in your answer.

[2]
Reveal mark scheme Mark scheme for question 4.4
4.5

Describe one way the method could be changed to obtain more accurate results for the rate of photosynthesis.

[1]
Reveal mark scheme Mark scheme for question 4.5
4.6

A gardener noticed that some plants were not growing as well as usual.

Describe two ways the gardener could identify a plant disease.

[2]
Reveal mark scheme Mark scheme for question 4.6
4.7

One of the plants had purple spots on its leaves and the leaves fell off.

What disease is the plant most likely to have?

[1]
Reveal mark scheme Mark scheme for question 4.7
4.8

A different plant had yellow leaves, but no spots.

Explain why plants may develop yellow leaves.

[2]
Reveal mark scheme Mark scheme for question 4.8

Paper 1 June 2024 Higher tier, Question 7 17 marks

Also involves Light intensity and the inverse square law

A student investigated the effect of different factors on photosynthesis.

The student used three leaves growing on the same plant.

Each leaf was treated in a different way.

After 48 hours the student tested each leaf for starch.

Table 3 shows the results.

Figure for question 7
7.1

Explain the results for the three leaves.

[5]
Reveal mark scheme Mark scheme for question 7.1
7.2

In another investigation the student used a different type of plant.

The plant was left uncovered in the light for 48 hours.

After 48 hours the student tested a leaf from the plant for starch.

Figure 6 shows the leaf before it was tested for starch.

Figure for question 7

Complete Table 4 to show the results you would expect for the starch test on the leaf in Figure 6.

Figure for question 7
[1]
Reveal mark scheme Mark scheme for question 7.2
7.3

Explain the results you gave in Question 07.2.

[2]
Reveal mark scheme Mark scheme for question 7.3
7.4

In some leaves, the green parts become yellow because of an ion deficiency.

Which ion is deficient in a plant with yellow leaves?

[1]
Reveal mark scheme Mark scheme for question 7.4
7.5

Give the scientific term that describes the yellow colour of the leaves.

[1]
Reveal mark scheme Mark scheme for question 7.5
7.6

The rate of photosynthesis is affected by different factors.

How could the oxygen produced during photosynthesis be used to measure the rate of photosynthesis?

[1]
Reveal mark scheme Mark scheme for question 7.6
7.7

Light, carbon dioxide and temperature are limiting factors of photosynthesis.

Figure 7 shows how the rate of photosynthesis is affected by light, carbon dioxide and temperature.

Figure for question 7

At point A on Figure 7, light is a limiting factor.

What is meant by a ‘limiting factor’?

[1]
Reveal mark scheme Mark scheme for question 7.7
7.8

Explain the effect of increasing temperature and increasing carbon dioxide concentration on the rate of photosynthesis shown in Figure 7.

[4]
Reveal mark scheme Mark scheme for question 7.8
7.9

Photosynthesis investigations often use a light source.

The spreading out of light from a source obeys the inverse square law.

The inverse square law links light intensity to distance from the light source.

Which of the following shows the inverse square law?

Tick (✓) one box.

Figure for question 7
[1]
Reveal mark scheme Mark scheme for question 7.9

Paper 1 June 2023 Foundation tier, Question 3 15 marks

Also involves Gas exchange · Sampling and fieldwork

Photosynthesis produces oxygen.

3.1

Complete the word equation for photosynthesis.

Choose answers from the box.

Figure for question 3

_____________________ + _____________________ → _____________________ + oxygen

[3]
Reveal mark scheme Mark scheme for question 3.1
3.2

Explain how oxygen is used in cells.

[2]
Reveal mark scheme Mark scheme for question 3.2
3.3

A student investigated the effect of light from different coloured light bulbs on photosynthesis.

The student:

• used pondweed in a beaker of water

• used different coloured light bulbs in a lamp

• counted the number of bubbles of oxygen the pondweed produced in 2 minutes for each colour of light bulb.

Give one hazard the student would need to consider when using the apparatus in this investigation.

Give the risk the hazard would cause.

[2]
Reveal mark scheme Mark scheme for question 3.3
3.4

The student needed to keep the temperature of the water in the beaker the same throughout the investigation.

Describe how the student could keep the temperature of the water the same.

[1]
Reveal mark scheme Mark scheme for question 3.4
3.5

The beaker of water contained the pondweed.

Explain why the temperature of the water in the beaker needed to be kept the same throughout the investigation.

[2]
Reveal mark scheme Mark scheme for question 3.5
3.6

Table 3 shows the results.

Figure for question 3

Which colour of light caused the highest rate of photosynthesis in the pondweed?

Tick (✓) one box.

Figure for question 3
[1]
Reveal mark scheme Mark scheme for question 3.6
3.7

What is the best way to display the data in Table 3 ?

Tick (✓) one box.

Figure for question 3
[1]
Reveal mark scheme Mark scheme for question 3.7
3.8

The student wanted to measure the volume of oxygen the pondweed produced in 2 minutes.

Name one piece of apparatus the student could use to measure the volume of oxygen.

[1]
Reveal mark scheme Mark scheme for question 3.8
3.9

Another student investigated the effect of light intensity on the rate of photosynthesis.

Figure 1 shows the results.

Figure for question 3

Describe what Figure 1 shows about the relationship between light intensity and the rate of photosynthesis.

[2]
Reveal mark scheme Mark scheme for question 3.9

Paper 1 June 2023 Higher tier, Question 6 15 marks

Also involves Osmosis

This question is about photosynthesis.

6.1

Complete the symbol equation for photosynthesis.

Question 6, shown as an image
[1]
Reveal mark scheme Mark scheme for question 6.1
6.2

Figure 8 shows how the rate of photosynthesis changes with light intensity.

Figure for question 6

Which part of the graph could be represented by the equation y = mx + c ? Tick (✓) one box.

Figure for question 6
[1]
Reveal mark scheme Mark scheme for question 6.2
6.3

A student investigated the effect of colour of light on the rate of photosynthesis

in leaves. Figure 9 shows how the investigation was set up.

Figure for question 6

Table 5 shows the results.

Figure for question 6

Give one way the student could change the colour of the light shining on the leaf discs.

[1]
Reveal mark scheme Mark scheme for question 6.3
6.4

Give the independent variable and the dependent variable in this investigation. Independent variable

[2]
Reveal mark scheme Mark scheme for question 6.4
6.5

Dependent variable

All of the air had to be removed from the leaf discs before placing them in the beaker. Suggest one reason why.

[1]
Reveal mark scheme Mark scheme for question 6.5
6.6

The leaf discs were placed in a beaker of sodium hydrogencarbonate

Question 6, shown as an image

Explain why sodium hydrogencarbonate solution was used instead of water.

[2]
Reveal mark scheme Mark scheme for question 6.6
6.7

Explain why the leaf discs moved to the surface of the solution during the investigation.

[2]
Reveal mark scheme Mark scheme for question 6.7
6.8

There are two types of chlorophyll in leaves.

Figure 10 shows the percentage of different wavelengths of light that the two types of chlorophyll absorb.

Figure for question 6

Table 6 shows the colour of different wavelengths of light.

Figure for question 6

Suggest the advantage to a plant of having two types of chlorophyll.

[1]
Reveal mark scheme Mark scheme for question 6.8
6.9

Table 5 is repeated below.

Figure for question 6

The leaf discs in the investigation are green. Explain the results in Table 5 for blue light and for green light. Use data from Figure 10 and Table 6.

[4]
Reveal mark scheme Mark scheme for question 6.9

Paper 1 June 2022 Foundation tier, Question 3 8 marks

Also involves Sampling and fieldwork

A scientist investigated the rate of photosynthesis of one type of tomato plant.

The tomato plants were grown in a greenhouse.

Table 2 shows the results.

Figure for question 3
3.1

Give two control variables the scientist should have used in the investigation.

[2]
Reveal mark scheme Mark scheme for question 3.1
3.2

Which range of carbon dioxide concentrations caused the rate of photosynthesis to change the most?

Tick (✓) one box.

Figure for question 3
[1]
Reveal mark scheme Mark scheme for question 3.2
3.3

How could the scientist have improved the validity of the results?

Tick (✓) one box.

Figure for question 3
[1]
Reveal mark scheme Mark scheme for question 3.3
3.4

Explain the change in the rate of photosynthesis when the concentration of carbon dioxide increased between 0.00% to 0.08%.

[2]
Reveal mark scheme Mark scheme for question 3.4
3.5

A farmer decided not to use a concentration of carbon dioxide higher than 0.08% to grow tomato plants.

Suggest two reasons for the farmer’s decision.

Use information from Table 2 and your own knowledge.

[2]
Reveal mark scheme Mark scheme for question 3.5

Paper 1 November 2021 Foundation tier, Question 2 14 marks

Also involves Cell structure · Virus lifecycle

Viruses cause disease.

2.1

What name is given to microorganisms that cause disease? Tick (✓) one box.

Figure for question 2
[1]
Reveal mark scheme Mark scheme for question 2.1
2.2

How do viruses cause the symptoms of disease? Tick (✓) one box.

Figure for question 2
[1]
Reveal mark scheme Mark scheme for question 2.2
2.3

Figure 4 shows a virus and an animal cell.

Figure for question 2

Suggest one reason why viruses are not classed as cells.

[1]
Reveal mark scheme Mark scheme for question 2.3
2.4

A vaccine can protect humans from a viral disease.

What does the vaccine contain? Tick (✓) one box.

Figure for question 2
[1]
Reveal mark scheme Mark scheme for question 2.4
2.5

In some cases, a first vaccination needs to be followed by a second vaccination some time later.

Which graph shows how the concentration of antibodies in a person’s blood changes after the first and second vaccinations? Tick (✓) one box.

Figure for question 2
[1]
Reveal mark scheme Mark scheme for question 2.5
2.6

Tobacco mosaic virus (TMV) causes disease in plants.

TMV affects the rate of photosynthesis in plants.

Which part of a plant shows discolouration caused by TMV? Tick (✓) one box.

Figure for question 2
[1]
Reveal mark scheme Mark scheme for question 2.6
2.7

Table 1 shows the rate of photosynthesis in four different tobacco plants.

Figure for question 2

Complete Figure 5. You should:

• label the y-axis

• add the correct scale to the y-axis

• plot the data from Table 1

• label each bar.

Figure for question 2
[5]
Reveal mark scheme Mark scheme for question 2.7
2.8

What conclusion can be made from the data in Table 1 ?

[1]
Reveal mark scheme Mark scheme for question 2.8
2.9

Explain why a high level of TMV infection reduces growth in a plant.

[2]
Reveal mark scheme Mark scheme for question 2.9

Paper 1 November 2020 Foundation tier, Question 7 16 marks

Also involves Sampling and fieldwork

This question is about photosynthesis.

7.1

Complete the word equation for photosynthesis.

Question 7, shown as an image
[2]
Reveal mark scheme Mark scheme for question 7.1
7.2

Describe how energy for the photosynthesis reaction is gained by plants.

[2]
Reveal mark scheme Mark scheme for question 7.2
7.3

Students investigated the effect of temperature on the rate of photosynthesis. The students shone light from a lamp onto pondweed and measured the volume of oxygen produced per hour. Table 3 shows the results.

Figure for question 7

Calculate mean value X.

[2]
Reveal mark scheme Mark scheme for question 7.3
7.4
Question 7, shown as an image

The students identified one anomalous result in Table 3.

Draw a ring around the anomalous result in Table 3.

[1]
Reveal mark scheme Mark scheme for question 7.4
7.5

Suggest one possible cause of the anomalous result.

[1]
Reveal mark scheme Mark scheme for question 7.5
7.6

How did the students deal with the anomalous result?

[1]
Reveal mark scheme Mark scheme for question 7.6
7.7

Give one factor the students should have kept constant in this investigation.

[1]
Reveal mark scheme Mark scheme for question 7.7
7.8

Table 3 is repeated below.

Figure for question 7

Why did the rate of photosynthesis decrease from 35 °C to 45 °C?

[1]
Reveal mark scheme Mark scheme for question 7.8
7.9

Complete Figure 10 using data from Table 3. You should:

• label the y-axis

• use a suitable scale for the y-axis

• plot the mean data from Table 3 for temperatures from 25 °C to 45 °C

• draw a line of best fit.

Figure for question 7
[5]
Reveal mark scheme Mark scheme for question 7.9

Paper 1 November 2020 Higher tier, Question 1 16 marks

This question is about photosynthesis.

1.1

Complete the word equation for photosynthesis.

Question 1, shown as an image
[2]
Reveal mark scheme Mark scheme for question 1.1
1.2

Describe how energy for the photosynthesis reaction is gained by plants.

[2]
Reveal mark scheme Mark scheme for question 1.2
1.3

Students investigated the effect of temperature on the rate of photosynthesis. The students shone light from a lamp onto pondweed and measured the volume of oxygen produced per hour. Table 1 shows the results.

Figure for question 1

Calculate mean value X.

[2]
Reveal mark scheme Mark scheme for question 1.3
1.4
Question 1, shown as an image

The students identified one anomalous result in Table 1.

Draw a ring around the anomalous result in Table 1.

[1]
Reveal mark scheme Mark scheme for question 1.4
1.5

Suggest one possible cause of the anomalous result.

[1]
Reveal mark scheme Mark scheme for question 1.5
1.6

How did the students deal with the anomalous result?

[1]
Reveal mark scheme Mark scheme for question 1.6
1.7

Give one factor the students should have kept constant in this investigation.

[1]
Reveal mark scheme Mark scheme for question 1.7
1.8

Table 1 is repeated below.

Figure for question 1

Why did the rate of photosynthesis decrease from 35 °C to 45 °C?

[1]
Reveal mark scheme Mark scheme for question 1.8
1.9

Complete Figure 1 using data from Table 1. You should:

• label the y-axis

• use a suitable scale for the y-axis

• plot the mean data from Table 1 for temperatures from 25 °C to 45 °C

• draw a line of best fit.

Figure for question 1
[5]
Reveal mark scheme Mark scheme for question 1.9

Paper 1 June 2019 Foundation tier, Question 9 12 marks

This question is about photosynthesis.

9.1

Complete the word equation for photosynthesis:

Question 9, shown as an image
[2]
Reveal mark scheme Mark scheme for question 9.1
9.2

A student investigated photosynthesis using pondweed.

Figure 14 shows the apparatus the student used.

Figure for question 9

This is the method used.

1. Set up the apparatus as shown in Figure 14.

2. Switch on the lamp.

3. After 20 minutes, record the volume of oxygen collected in the measuring cylinder.

4. Repeat steps 1–3 using bulbs of different power output.

What was the independent variable in the investigation?

Tick (✓) one box.

Figure for question 9
[1]
Reveal mark scheme Mark scheme for question 9.2
9.3

Suggest two ways the method could be improved so the results would be more valid.

[2]
Reveal mark scheme Mark scheme for question 9.3
9.4

Table 9 shows the student’s results.

Figure for question 9

Calculate value X in Table 9.

Question 9, shown as an image
[1]
Reveal mark scheme Mark scheme for question 9.4
9.5

Complete Figure 15.

You should:

• label the x-axis

• use a suitable scale

• plot the data from Table 9 and your answer to Question 09.4

• draw a line of best fit.

Figure for question 9
[4]
Reveal mark scheme Mark scheme for question 9.5
9.6

Determine the expected rate of photosynthesis with a bulb of power output 75 watts.

Use Figure 15.

Question 9, shown as an image
[1]
Reveal mark scheme Mark scheme for question 9.6
9.7

Which graph shows the effect of temperature on the rate of photosynthesis?

Tick (✓) one box.

Figure for question 9
[1]
Reveal mark scheme Mark scheme for question 9.7

Paper 1 June 2019 Higher tier, Question 3 12 marks

Also involves Light intensity and the inverse square law

This question is about photosynthesis.

3.1

Complete the word equation for photosynthesis:

Question 3, shown as an image
[2]
Reveal mark scheme Mark scheme for question 3.1
3.2

A student investigated photosynthesis using pondweed.

Figure 3 shows the apparatus the student used.

Figure for question 3

This is the method used.

1. Set up the apparatus as shown in Figure 3.

2. Switch on the lamp.

3. After 20 minutes, record the volume of oxygen collected in the measuring cylinder.

4. Repeat steps 1–3 using bulbs of different power output.

What was the independent variable in the investigation?

Tick (✓) one box.

Figure for question 3
[1]
Reveal mark scheme Mark scheme for question 3.2
3.3

Suggest two ways the method could be improved so the results would be more valid.

[2]
Reveal mark scheme Mark scheme for question 3.3
3.4

Table 3 shows the student’s results.

Figure for question 3

Calculate value X in Table 3.

Question 3, shown as an image
[1]
Reveal mark scheme Mark scheme for question 3.4
3.5

Complete Figure 4.

You should:

• label the x-axis

• use a suitable scale

• plot the data from Table 3 and your answer to Question 03.4

• draw a line of best fit.

Figure for question 3
[4]
Reveal mark scheme Mark scheme for question 3.5
3.6

Determine the expected rate of photosynthesis with a bulb of power output 75 watts.

Use Figure 4.

Question 3, shown as an image
[1]
Reveal mark scheme Mark scheme for question 3.6
3.7

Which graph shows the effect of temperature on the rate of photosynthesis?

Tick (✓) one box.

Figure for question 3
[1]
Reveal mark scheme Mark scheme for question 3.7

Paper 1 June 2018 Higher tier, Question 5 10 marks

Plants can be infected by fungi, viruses and insects.

Aphids are small insects that carry pathogens.

Figure 6 shows an aphid feeding from a plant stem.

Figure for question 5
5.1

An aphid feeds by inserting its sharp mouthpiece into the stem of a plant.

Give the reason why the mouthpiece of an aphid contains a high concentration of dissolved sugars after feeding.

[1]
Reveal mark scheme Mark scheme for question 5.1
5.2

Plants infected with aphids may show symptoms of magnesium deficiency.

Magnesium deficiency symptoms include:

• yellow leaves

• stunted growth.

Explain how a deficiency of magnesium could cause these symptoms.

[5]
Reveal mark scheme Mark scheme for question 5.2
5.3

A farmer thinks a potato crop is infected with potato virus Y (PVY).

The farmer obtains a monoclonal antibody test kit for PVY.

To make the monoclonal antibodies a scientist first isolates the PVY protein from the virus.

Describe how the scientist would use the protein to produce the PVY monoclonal antibody.

[4]
Reveal mark scheme Mark scheme for question 5.3

Report a problem with a question on this page
What is wrong?

Goes to whoever maintains the question split. No account needed and nothing is published on this page.

Memes

Certified Biology memes. Peer-reviewed by nobody.

Laugh now. Cry in the exam hall later. Then come back and do a past paper.

Learn the whole of osmosis
Write ‘water moves’ and leave
Me on the 6-marker about potatoes
Knowing the mitochondria is the powerhouse
Knowing what ATP stands for
Drawing the heart the right way round
Explaining active transport without crying
Biology enlightenment, ranked

Mum 💖

how's biology going?? 🥰

learning about the heart rn

that's my baby 💖

📱 Screen time this week · Snapchat5h 52m

The heart, in fact, was broken

Biology starter pack

Microscope you can't focusPotato chips in salt waterCress that died in week 1This website
Collect all four