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Edexcel GCSE Biology

Topic questions Ecology Abiotic & biotic factors

Abiotic & biotic factors

14 questions · 139 marks · about 2.5 hours

Abiotic and biotic factors questions

Edexcel 1BI0 Abiotic and biotic factors 14 questions

Paper 2 June 2025 Foundation tier, Question 9 13 marks

(a)

In 2018, all the plants were removed from the soil in a 1000 m2 area.

A wall was built around this area to keep people out.

In 2019, three species of plants were recorded growing in this area.

Two of these species were indigenous.

(a)(i)

State what is meant by the term ’indigenous’.

[1]
Reveal mark scheme Mark scheme for question 9(a)(i)
(a)(ii)

Name one piece of equipment that could be used to sample the number of indigenous plants in this area.

[1]
Reveal mark scheme Mark scheme for question 9(a)(ii)
(b)

The area was surveyed every year and the number of plants of each species (A to H) was estimated.

Figure 20 shows the results.

Figure for question 9
(b)(i)

State the trend shown by the data in Figure 20 for plant species C from 2019 to 2023.

[1]
Reveal mark scheme Mark scheme for question 9(b)(i)
(b)(ii)

A scientist suggested that the decrease in the number of plants of species B in 2021 was caused by competition from one of the other species of plant.

Which species is most likely to have out-competed species B in 2021?

Question 9, shown as an image
[1]
Reveal mark scheme Mark scheme for question 9(b)(ii)
(b)(iii)

Explain one way that this species could have out‑competed species B.

[2]
Reveal mark scheme Mark scheme for question 9(b)(iii)
(b)(iv)

Give one way that species H could have moved into the area.

[1]
Reveal mark scheme Mark scheme for question 9(b)(iv)
(c)

*(c) Figure 21 shows some plants growing in a small area of a 1000 m2 field.

Figure for question 9

Devise a plan to estimate the number of named plant species growing in this field.

[6]
Reveal mark scheme Mark scheme for question 9(c)

Paper 2 June 2025 Higher tier, Question 3 9 marks

(a)

In 2018, all the plants were removed from the soil in a 1000 m2 area.

A wall was built around this area to keep people out.

In 2019, three species of plants were recorded growing in this area.

Two of these species were indigenous.

(a)(i)

State what is meant by the term ’indigenous’.

[1]
Reveal mark scheme Mark scheme for question 3(a)(i)
(a)(ii)

Name one piece of equipment that could be used to sample the number of indigenous plants in this area.

[1]
Reveal mark scheme Mark scheme for question 3(a)(ii)
(b)

The area was surveyed every year and the number of plants of each species (A to H) was estimated.

Figure 6 shows the results.

Figure for question 3
(b)(i)

State the trend shown by the data in Figure 6 for plant species C from 2019 to 2023.

[1]
Reveal mark scheme Mark scheme for question 3(b)(i)
(b)(ii)

A scientist suggested that the decrease in the number of plants of species B in 2021 was caused by competition from one of the other species of plant.

Which species is most likely to have out-competed species B in 2021?

Question 3, shown as an image
[1]
Reveal mark scheme Mark scheme for question 3(b)(ii)
(b)(iii)

Explain one way that this species could have out‑competed species B.

[2]
Reveal mark scheme Mark scheme for question 3(b)(iii)
(b)(iv)

Give one way that species H could have moved into the area.

[1]
Reveal mark scheme Mark scheme for question 3(b)(iv)
(c)

Plants also need water to grow.

Explain how water is absorbed into the plant.

[2]
Reveal mark scheme Mark scheme for question 3(c)

Paper 2 June 2025 Higher tier, Question 7 10 marks

Also involves Food security

(a)

Figure 13 shows the biomass of each population of organisms in a food chain.

Question 7, shown as an image
Question 7, shown as an image

Figure 13

(a)(i)

Calculate the percentage efficiency of biomass transfer from the aphids to the ladybirds.

Use the equation:

Question 7, shown as an image

Give your answer to the nearest whole number.

[3]
Reveal mark scheme Mark scheme for question 7(a)(i)
(a)(ii)

Give two reasons why the biomass of ladybirds is lower than the biomass of aphids in this food chain.

[2]
Reveal mark scheme Mark scheme for question 7(a)(ii)
(a)(iii)

Aphids are a problem for rose growers as they damage the leaves of rose plants.

Explain how ladybirds could be used to reduce the number of aphids.

[2]
Reveal mark scheme Mark scheme for question 7(a)(iii)
(b)

As human population rises, food security is an increasing problem throughout the world.

Give three factors that reduce the amount of food available for humans throughout the world.

[3]
Reveal mark scheme Mark scheme for question 7(b)

Paper 2 June 2024 Foundation tier, Question 3 8 marks

Also involves Global warming

Figure 4 shows a food chain in an oak woodland.

oak tree fraction aphids fraction ladybirds fraction robins

Question 3, shown as an image
(a)

State what the arrows in the food chain represent.

[1]
Reveal mark scheme Mark scheme for question 3(a)
(b)
Figure for question 3
[1]
Reveal mark scheme Mark scheme for question 3(b)
(c)

A robin in this woodland eats 6 g of food in a day.

The robin uses 15% of this food for growth.

Calculate the mass of food that is used for growth.

[2]
Reveal mark scheme Mark scheme for question 3(c)
(d)

Temperatures in England have increased over the last 20 years.

(d)(i)

State two human activities that have caused this temperature increase.

[2]
Reveal mark scheme Mark scheme for question 3(d)(i)
(d)(ii)

One new species of moth is now able to live in England because of the increased temperatures.

This moth feeds on oak leaves.

Explain how these moths may affect the number of aphids in the food chain shown in Figure 4.

[2]
Reveal mark scheme Mark scheme for question 3(d)(ii)

Paper 2 June 2023 Foundation tier, Question 6 11 marks

6 In 2000, a council planted indigenous trees as part of a reforestation programme.

(a)

(a) Why were indigenous trees used instead of non-indigenous trees?

Question 6, shown as an image
[1]
Reveal mark scheme Mark scheme for question 6(a)
(b)

(b) The animal biodiversity in this reforested area was measured from 2004 to 2017. Biodiversity is measured on a scale from 0 to 1. 0 = low biodiversity 1 = maximum biodiversity Figure 12 shows the results.

Figure for question 6

2000 2002 2004 2006 2008 2010 2012 2014 2016 2018 2020 2022

Figure 12

(b)(i)

(i) Draw a straight line of best fit on Figure 12 to show the main trend in this data.

[1]
Reveal mark scheme Mark scheme for question 6(b)(i)
(b)(ii)

(ii) Estimate the animal biodiversity of the reforested area in 2022.

[1]
Reveal mark scheme Mark scheme for question 6(b)(ii)
(c)
(c)(i)

(c) (i) The data to calculate animal biodiversity was collected using the following method.

• a 100 m2 area was chosen

• three 1 m2 quadrats were placed randomly on the ground

• animals inside the quadrat were counted

• the number of each species of animal was recorded

State two ways that the quality of the results from this method could be improved.

[2]
Reveal mark scheme Mark scheme for question 6(c)(i)
(c)(ii)

(ii) State why point A on Figure 12 was ignored when considering the trend in animal biodiversity.

[1]
Reveal mark scheme Mark scheme for question 6(c)(ii)
(c)(iii)

(iii) The data shown in Figure 12 was collected during the summer. Explain why animal biodiversity would be different if the data had been collected in the winter.

[2]
Reveal mark scheme Mark scheme for question 6(c)(iii)
(d)

(d) A scientist observes that trees at the edge of a forest shade part of a field. Describe how a belt transect could be used to see how light intensity affects the biodiversity of plants growing in the field.

[3]
Reveal mark scheme Mark scheme for question 6(d)

Paper 2 June 2023 Higher tier, Question 1 7 marks

Figure 1 shows a stream flowing near a fertiliser factory.

The factory burns coal as a source of energy.

The factory releases waste into the stream and sulfur dioxide into the air.

Samples of water were taken at five points, A, B, C, D and E, as shown on Figure 1.

Figure for question 1

Figure 2 shows the oxygen concentration in the water at the five points along the stream.

Figure for question 1
(a)
(a)(i)

Calculate how many times greater the oxygen concentration is at point E than at point A.

[1]
Reveal mark scheme Mark scheme for question 1(a)(i)
(a)(ii)

State how the oxygen concentration changes from point A to point E.

[1]
Reveal mark scheme Mark scheme for question 1(a)(ii)
(a)(iii)

Which indicator species would be most likely to be seen in the water at point A?

Question 1, shown as an image
[1]
Reveal mark scheme Mark scheme for question 1(a)(iii)
(a)(iv)

Explain where the biodiversity will be highest in the stream.

[3]
Reveal mark scheme Mark scheme for question 1(a)(iv)
(b)

Figure 3 shows rose leaves infected with blackspot fungus.

Figure for question 1

(Source: © Manfred Ruckszio/Shutterstock)

Figure 3

State why rose plants growing near this factory are not infected with blackspot fungus.

[1]
Reveal mark scheme Mark scheme for question 1(b)

Paper 2 June 2022 Foundation tier, Question 10 11 marks

Also involves Trophic levels

10 A student investigated the width of leaves on nettle plants growing in two areas next to a woodland. Figure 19a shows a nettle plant and Figure 19b shows a map of the woodland showing area A and area B. The woodland caused area A to be in the shade.

Figure for question 10

(Source: © Alila Medical Media/Shutterstock) Figure 19a Figure 19b The student measured the maximum width of leaves on five plants from each area. The student always measured one leaf from the fourth pair of leaves.

(a)

(a) Give one reason why the student always measured a leaf from the fourth pair of leaves.

[1]
Reveal mark scheme Mark scheme for question 10(a)
(b)

(b) Figure 20 shows the results.

Figure for question 10 Figure for question 10

Figure 20

(b)(i)

(i) Why did the student not include the circled width when calculating the mean for area B?

Question 10, shown as an image
[1]
Reveal mark scheme Mark scheme for question 10(b)(i)
(b)(ii)

(ii) Explain the difference in the mean width of leaves in the shade and those in the sunlight.

[2]
Reveal mark scheme Mark scheme for question 10(b)(ii)
(c)

(c) The student also studied some of the animals in areas A and B. The student saw caterpillars eating the leaves of some nettles. The student also saw a toad eating a large beetle. Large beetles eat ladybirds. Ladybirds eat caterpillars.

(c)(i)

(i) Give the food chain for these feeding relationships.

[3]
Reveal mark scheme Mark scheme for question 10(c)(i)
(c)(ii)

(ii) Frogs also eat large beetles. Figure 21 shows the energy transferred between these animals.

Figure for question 10

Figure 21

Calculate the percentage efficiency of energy transfer from the large beetles to the frog.

[2]
Reveal mark scheme Mark scheme for question 10(c)(ii)
(c)(iii)

(iii) Give two reasons why only some of the energy in the biomass of the large beetles is transferred to the biomass of the frog.

[2]

Paper 2 June 2022 Higher tier, Question 4 11 marks

Also involves Photosynthesis · Trophic levels

4 A student investigated the width of leaves on nettle plants growing in two areas next to a woodland. Figure 5a shows a nettle plant and Figure 5b shows a map of the woodland showing area A and area B. The woodland caused area A to be in the shade.

Figure for question 4

(Source: © Alila Medical Media/Shutterstock) Figure 5a Figure 5b The student measured the maximum width of leaves on five plants from each area. The student always measured one leaf from the fourth pair of leaves.

(a)

(a) Give one reason why the student always measured a leaf from the fourth pair of leaves.

[1]
Reveal mark scheme Mark scheme for question 4(a)
(b)

(b) Figure 6 shows the results.

Figure for question 4 Figure for question 4
(b)(i)

(i) Why did the student not include the circled width when calculating the mean for area B?

Question 4, shown as an image
[1]
Reveal mark scheme Mark scheme for question 4(b)(i)
(b)(ii)

(ii) Explain the difference in the mean width of leaves in the shade and those in the sunlight.

[2]
Reveal mark scheme Mark scheme for question 4(b)(ii)
(c)

(c) The student also studied some of the animals in areas A and B. The student saw caterpillars eating the leaves of some nettles. The student also saw a toad eating a large beetle. Large beetles eat ladybirds. Ladybirds eat caterpillars.

(c)(i)

(i) Give the food chain for these feeding relationships.

[3]
Reveal mark scheme Mark scheme for question 4(c)(i)
(c)(ii)

(ii) Frogs also eat large beetles. Figure 7 shows the energy transferred between these animals.

Figure for question 4

Calculate the percentage efficiency of energy transfer from the large beetles to the frog.

[2]
Reveal mark scheme Mark scheme for question 4(c)(ii)
(c)(iii)

(iii) Give two reasons why only some of the energy in the biomass of the large beetles is transferred to the biomass of the frog.

[2]
Reveal mark scheme Mark scheme for question 4(c)(iii)

Paper 2 November 2021 Foundation tier, Question 8 11 marks

Figure 16 shows an area of woodland with some small plants growing in the ground between the trees.

Figure for question 8

(Source: © Maksym Holovinov/Shutterstock)

Figure 16

(a)

The leaves of the small plants are green.

Describe how these plants make glucose.

[2]
Reveal mark scheme Mark scheme for question 8(a)
(b)

Name a group of organisms that break down the dead leaves and release mineral ions into the soil.

[1]
Reveal mark scheme Mark scheme for question 8(b)
(c)

The mineral ions are absorbed from the soil into the roots of plants.

Describe how these mineral ions are transported from the roots to the leaves of the plants.

[2]
Reveal mark scheme Mark scheme for question 8(c)
(d)

A scientist recorded the mean light intensity and the mean number of small plants per m2 for six 25 m2 areas of the woodland.

Figure 17 shows the results.

Figure for question 8
(d)(i)

Explain the effect of light intensity on the number of small plants per m2.

[2]
Reveal mark scheme Mark scheme for question 8(d)(i)
(d)(ii)

State one variable the scientist should have controlled to make sure the light intensity measurements could be compared.

[1]
Reveal mark scheme Mark scheme for question 8(d)(ii)
(e)

The scientist selects an area near the edge of the woodland where many stinging nettles are growing.

This area is partly shaded by the trees.

Describe how the scientist should use a belt transect to investigate how light intensity affects the growth of stinging nettles.

[3]
Reveal mark scheme Mark scheme for question 8(e)

Paper 2 November 2021 Higher tier, Question 1 7 marks

The population of native white‑clawed crayfish in English rivers is decreasing due to an increasing population of American imported signal crayfish.

Figure 1 shows a white‑clawed crayfish.

Figure for question 1
(i)

Which term is used to describe the American imported signal crayfish now that it is living in English rivers?

Question 1, shown as an image
[1]
Reveal mark scheme Mark scheme for question 1(i)
(ii)

American imported signal crayfish may eat white‑clawed crayfish.

Explain one other reason why the increasing population of American imported signal crayfish may have caused the decrease in white‑clawed crayfish.

[2]
Reveal mark scheme Mark scheme for question 1(ii)
(iii)

The Environment Agency is developing methods of increasing the population of white‑clawed crayfish in English rivers.

Which term describes these methods?

Question 1, shown as an image
[1]
Reveal mark scheme Mark scheme for question 1(iii)
(iv)

White‑clawed crayfish cannot survive in rivers with a low concentration of oxygen.

Explain how eutrophication can cause the population of white‑clawed crayfish to decrease.

[3]
Reveal mark scheme Mark scheme for question 1(iv)

Paper 2 November 2021 Higher tier, Question 2 9 marks

Also involves Photosynthesis

Figure 2 shows an area of woodland with some small plants growing in the ground between the trees.

Figure for question 2

(Source: © Maksym Holovinov/Shutterstock)

Figure 2

(a)

Name a group of organisms that break down the dead leaves and release mineral ions into the soil.

[1]
Reveal mark scheme Mark scheme for question 2(a)
(b)

The mineral ions are absorbed from the soil into the roots of plants.

Describe how these mineral ions are transported from the roots to the leaves of the plants.

[2]
Reveal mark scheme Mark scheme for question 2(b)
(c)

A scientist recorded the mean light intensity and the mean number of small plants per m2 for six 25 m2 areas of the woodland.

Figure 3 shows the results.

Figure for question 2
(c)(i)

Explain the effect of light intensity on the number of small plants per m2.

[2]
Reveal mark scheme Mark scheme for question 2(c)(i)
(c)(ii)

State one variable the scientist should have controlled to make sure the light intensity measurements could be compared.

[1]
Reveal mark scheme Mark scheme for question 2(c)(ii)
(d)

The scientist selects an area near the edge of the woodland where many stinging nettles are growing.

This area is partly shaded by the trees.

Describe how the scientist should use a belt transect to investigate how light intensity affects the growth of stinging nettles.

[3]
Reveal mark scheme Mark scheme for question 2(d)

Paper 2 June 2019 Foundation tier, Question 5 10 marks

Figure 8 shows an area of nettle plants.

Figure for question 5

Grass does not grow among the nettles.

(a)

Explain why grass does not grow where there are nettles.

[2]
Reveal mark scheme Mark scheme for question 5(a)
(b)

Figure 9 shows caterpillars eating nettle leaves.

Figure for question 5

A caterpillar has a body mass of 6.0 grams. One week later, its body mass had increased to 7.5 grams. Caterpillars convert 10% of food eaten into body mass.

(b)(i)

Calculate the mass of nettles that the caterpillar ate.

[2]
Reveal mark scheme Mark scheme for question 5(b)(i)
(b)(ii)

Describe what happens to food eaten that is not converted into the body mass of the caterpillar.

[2]
Reveal mark scheme Mark scheme for question 5(b)(ii)
(c)

Devise a method a scientist could use to investigate how temperature affects nettle growth.

[4]
Reveal mark scheme Mark scheme for question 5(c)

Paper 2 June 2018 Foundation tier, Question 10 11 marks

Also involves Spread of disease

Since 2003, in France, people have been buying Siberian chipmunks as pets but then releasing them into the wild when they are no longer wanted.

They are now classified as an invasive species.

Figure 25 shows a Siberian chipmunk (Tamias sibiricus).

Figure for question 10

Figure 25

(a)

Siberian chipmunks eat acorns, which are the seeds of oak trees.

In Siberia, the natural predators of Siberian chipmunks are wild dogs.

(a)(i)

Figure 26 shows the biomass of three organisms in a food chain from one area of Siberia.

Figure for question 10

Draw a pyramid of biomass for this food chain.

Figure for question 10
[2]
Reveal mark scheme Mark scheme for question 10(a)(i)
(a)(ii)

In France, Siberian chipmunks have very few natural predators.

Describe how this affected the Siberian chipmunk population in France.

[2]
Reveal mark scheme Mark scheme for question 10(a)(ii)
(a)(iii)

The percentage of energy transferred from the acorns to the chipmunks is 9.5%.

The energy contained in the acorns is 97 500 kJ.

Calculate the amount of energy transferred to the chipmunks.

Give your answer to the nearest whole number.

[3]
Reveal mark scheme Mark scheme for question 10(a)(iii)
(b)

The black-legged tick (Ixodes scapularis) is a parasite that feeds on the blood of animals including Siberian chipmunks and humans.

The tick transmits the Lyme disease pathogen.

Figure 27 shows the number of cases of Lyme disease in humans in France in 2003 and 2015.

Figure for question 10
(b)(i)

Calculate the percentage increase in the number of cases of Lyme disease in humans in France from 2003 to 2015.

[2]
Reveal mark scheme Mark scheme for question 10(b)(i)
(b)(ii)

Explain why there has been an increase in the number of cases of Lyme disease in humans in France.

[2]
Reveal mark scheme Mark scheme for question 10(b)(ii)

Paper 2 June 2018 Higher tier, Question 4 11 marks

Also involves Spread of disease

Since 2003, in France, people have been buying Siberian chipmunks as pets but then releasing them into the wild when they are no longer wanted.

They are now classified as an invasive species.

Figure 7 shows a Siberian chipmunk (Tamias sibiricus).

Figure for question 4
(a)

Siberian chipmunks eat acorns which, are the seeds of oak trees.

In Siberia, the natural predators of Siberian chipmunks are wild dogs.

(a)(i)

Figure 8 shows the biomass of three organisms in a food chain from one area of Siberia.

Figure for question 4

Draw a pyramid of biomass for this food chain.

Figure for question 4
[2]
Reveal mark scheme Mark scheme for question 4(a)(i)
(a)(ii)

In France, Siberian chipmunks have very few natural predators.

Describe how this affected the Siberian chipmunk population in France.

[2]
Reveal mark scheme Mark scheme for question 4(a)(ii)
(a)(iii)

The percentage of energy transferred from the acorns to the chipmunks is 9.5%.

The energy contained in the acorns is 97 500 kJ.

Calculate the amount of energy transferred to the chipmunks.

Give your answer to the nearest whole number.

[3]
Reveal mark scheme Mark scheme for question 4(a)(iii)
(b)

The black-legged tick (Ixodes scapularis) is a parasite that feeds on the blood of animals including Siberian chipmunks and humans.

The tick transmits the Lyme disease pathogen.

Figure 9 shows the number of cases of Lyme disease in humans in France in 2003 and 2015.

Figure for question 4
(b)(i)

Calculate the percentage increase in the number of cases of Lyme disease in humans in France from 2003 to 2015.

[2]
Reveal mark scheme Mark scheme for question 4(b)(i)
(b)(ii)

Explain why there has been an increase in the number of cases of Lyme disease in humans in France.

[2]
Reveal mark scheme Mark scheme for question 4(b)(ii)

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