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Edexcel GCSE Biology

Topic questions Plant biology Transpiration

Transpiration

18 questions · 181 marks · about 3 hours

Transpiration questions

Edexcel 1BI0 Transpiration 18 questions

Paper 2 June 2025 Foundation tier, Question 5 9 marks

Also involves Plant hormones · Tropisms · Leaf structure

(a)

Figure 9 shows a stoma on the lower epidermis of a leaf as seen using a

Figure for question 5
(a)(i)

Draw the stoma and two cells around it in the box.

Figure for question 5
[2]
Reveal mark scheme Mark scheme for question 5(a)(i)
(a)(ii)

Give the name of the two cells around the stoma.

[1]
Reveal mark scheme Mark scheme for question 5(a)(ii)
(b)

A student observed that a plant in a room had grown towards the light.

(b)(i)

Name the plant hormone that causes the plant to grow towards the light.

[1]
Reveal mark scheme Mark scheme for question 5(b)(i)
(b)(ii)

Name the type of tropism that causes the plant to grow towards the light.

[1]
Reveal mark scheme Mark scheme for question 5(b)(ii)
(c)

Figure 10 compares the root hair cells of two plants.

Plant A is adapted to growing in wet soil.

Plant B is adapted to growing in dry soil.

Figure for question 5
(c)(i)

Compare the root hair cells of Plant A with the root hair cells of plant B.

[2]
Reveal mark scheme Mark scheme for question 5(c)(i)
(c)(ii)

Figure 11 shows sections of a leaf from plant A and a leaf from plant B.

Figure for question 5

plant A (wet soil)
plant B (dry soil)

Figure 11

State two differences in the leaf structure shown in Figure 11 that help plant B to survive in a dry environment.

[2]
Reveal mark scheme Mark scheme for question 5(c)(ii)

Paper 2 June 2025 Higher tier, Question 6 9 marks

Figure 12 shows the lower epidermis of a leaf, with a stoma labelled.

Figure for question 6

(Source: © Quagga Media/Alamy Stock Photo)

Figure 12

(a)
(a)(i)

State the number of guard cells shown in Figure 12.

[1]
Reveal mark scheme Mark scheme for question 6(a)(i)
(a)(ii)

State one gas that leaves the stomata and one gas that enters the stomata during daylight.

gas leaving stomata

gas entering stomata

[2]
Reveal mark scheme Mark scheme for question 6(a)(ii)
(a)(iii)

State why the stomata close.

[1]
Reveal mark scheme Mark scheme for question 6(a)(iii)
(b)

A student wanted to view the cross-section of a leaf on a prepared slide.

Describe how a light microscope would be used to observe this slide.

[3]
Reveal mark scheme Mark scheme for question 6(b)
(c)

Sugars are transported from the leaves to the rest of the plant.

Explain how sugars are moved through the plant.

[2]
Reveal mark scheme Mark scheme for question 6(c)

Paper 2 June 2024 Foundation tier, Question 5 9 marks

Also involves Leaf structure · Adaptations

Figure 7 shows the structure of a leaf.

Figure for question 5
(a)
(a)(i)

Which layer has the highest number of chloroplasts per cell?

Question 5, shown as an image
[1]
Reveal mark scheme Mark scheme for question 5(a)(i)
(a)(ii)

When iodine solution was put onto these cells, small parts of the cells became blue‑black.

Name the substance stored in the parts of the cells that turned blue‑black.

[1]
Reveal mark scheme Mark scheme for question 5(a)(ii)
(a)(iii)

State how the guard cells control the amount of gas exchange that occurs through the stomata.

[1]
Reveal mark scheme Mark scheme for question 5(a)(iii)
(b)

A scientist measured the thickness of the cuticle from the leaves of plants growing in soil that had a different percentage of water content.

The scientist measured six plants at each percentage of water content.

Figure 8 shows the results.

Figure for question 5
(b)(i)

Calculate the mean thickness of cuticle for the plants growing in soil containing 15% water.

Give your answer to one decimal place.

[3]
Reveal mark scheme Mark scheme for question 5(b)(i)
(b)(ii)

Explain why the thickness of the cuticle is different when a plant is grown in soil with a higher water content.

[2]
Reveal mark scheme Mark scheme for question 5(b)(ii)
(b)(iii)

State one other way that the leaves of plants are adapted to help plants grow in soil with low water content.

[1]
Reveal mark scheme Mark scheme for question 5(b)(iii)

Paper 2 June 2024 Foundation tier, Question 9 13 marks

(a)

Figure 13 shows a root hair cell from a plant.

Figure for question 9
(a)(i)

Name the part labelled X.

[1]
Reveal mark scheme Mark scheme for question 9(a)(i)
(a)(ii)

State one way that the structure of the root hair cell increases the volume of substances it absorbs.

[1]
Reveal mark scheme Mark scheme for question 9(a)(ii)
(a)(iii)

Explain why root hair cells do not contain chloroplasts.

[3]
Reveal mark scheme Mark scheme for question 9(a)(iii)
(b)
Question 9, shown as an image
Figure for question 9
Question 9, shown as an image
[2]
Reveal mark scheme Mark scheme for question 9(b)
(c)

*(c) The arrows in Figure 15 show the direction of water movement through a tree.

Figure for question 9

Explain how water is moved from the soil, through the plant and into the air.

[6]
Reveal mark scheme Mark scheme for question 9(c)

Paper 2 June 2024 Higher tier, Question 7 11 marks

Also involves Tropisms

7 A farmer wants to make sunflower seeds germinate faster.

(a)

(a) Which is the best hormone for the farmer to use?

Question 7, shown as an image
[1]
Reveal mark scheme Mark scheme for question 7(a)
(b)

(b) The sunflower plants grew and flowered. The farmer noticed that the flowers faced a different direction at different times of the day. Figure 8 shows a sunflower at different times of the day. This movement is an example of phototropism.

Figure for question 7

(Source: © yevgeniy11/Shutterstock)

Figure 8

(b)(i)

(i) This movement of the flower was in response to the light. Explain how the sunflower changed direction throughout the day.

[4]
Reveal mark scheme Mark scheme for question 7(b)(i)
(b)(ii)

(ii) Explain how water moves through the stem of the sunflower plant to the leaves.

[3]
Reveal mark scheme Mark scheme for question 7(b)(ii)
(b)(iii)

(iii) Sunflower leaves are large. Explain why large leaves are an advantage to the plant.

[3]
Reveal mark scheme Mark scheme for question 7(b)(iii)

Paper 2 June 2022 Foundation tier, Question 8 12 marks

(a)

8 (a) Figure 14 shows a diagram of a plant root hair cell.

Figure for question 8

Figure 14

(a)(i)

(i) Name the part labelled R.

[1]
Reveal mark scheme Mark scheme for question 8(a)(i)
(a)(ii)

(ii) Explain one adaptation of a root hair cell that increases the absorption of water and mineral ions.

[2]
Reveal mark scheme Mark scheme for question 8(a)(ii)
(b)

(b) Figure 15 shows xylem and phloem from the stem of a plant.

Figure for question 8

Figure 15

(b)(i)

(i) Living cells in phloem use energy to transport sucrose. Which organelles release energy in living cells?

Question 8, shown as an image
[1]
Reveal mark scheme Mark scheme for question 8(b)(i)
(b)(ii)

(ii) Describe two features of the structure of xylem vessels that can be seen in Figure 15.

[2]
Reveal mark scheme Mark scheme for question 8(b)(ii)
(c)

(c) A scientist investigated how the flow of air affected the rate of transpiration in a plant. A fan was used to change the flow of air. The volume of water taken up by the plant was measured. Figure 16 shows the results of this investigation. fan switched on

Figure for question 8
Question 8, shown as an image

time in minutes

Figure 16

(c)(i)

(i) Explain why switching on the fan caused a change in the volume of water taken up by the plant.

[3]
Reveal mark scheme Mark scheme for question 8(c)(i)
(c)(ii)

(ii) Give one reason why the volume of water taken up by the plant was also measured when the fan was not switched on.

[1]
Reveal mark scheme Mark scheme for question 8(c)(ii)
(c)(iii)

(iii) Calculate the rate of water uptake from 8 minutes to 10 minutes when the fan was switched on. Use the equation

Question 8, shown as an image

..............................................................mm3 per minute

[2]
Reveal mark scheme Mark scheme for question 8(c)(iii)

Paper 2 June 2022 Higher tier, Question 2 9 marks

(a)

2 (a) Figure 2 shows xylem and phloem from the stem of a plant.

Figure for question 2
(a)(i)

(i) Living cells in phloem use energy to transport sucrose. Which organelles release energy in living cells?

Question 2, shown as an image
[1]
Reveal mark scheme Mark scheme for question 2(a)(i)
(a)(ii)

(ii) Describe two features of the structure of xylem vessels that can be seen in Figure 2.

[2]
Reveal mark scheme Mark scheme for question 2(a)(ii)
(b)

(b) A scientist investigated how the flow of air affected the rate of transpiration in a plant. A fan was used to change the flow of air. The volume of water taken up by the plant was measured. Figure 3 shows the results of this investigation. fan switched on

Figure for question 2
Question 2, shown as an image

time in minutes

Figure 3

(b)(i)

(i) Explain why switching on the fan caused a change in the volume of water taken up by the plant.

[3]
Reveal mark scheme Mark scheme for question 2(b)(i)
(b)(ii)

(ii) Give one reason why the volume of water taken up by the plant was also measured when the fan was not switched on.

[1]
Reveal mark scheme Mark scheme for question 2(b)(ii)
(b)(iii)

(iii) Calculate the rate of water uptake from 8 minutes to 10 minutes when the fan was switched on. Use the equation

Question 2, shown as an image

..............................................................mm3 per minute

[2]
Reveal mark scheme Mark scheme for question 2(b)(iii)

Paper 2 November 2021 Foundation tier, Question 7 12 marks

Also involves Tropisms · Leaf structure

Figure 12 shows how the leaves are arranged on a plant.

Figure for question 7
(a)

Which reason explains why the leaves are arranged in this way?

Question 7, shown as an image
[1]
Reveal mark scheme Mark scheme for question 7(a)
(b)

Figure 13 shows guard cells and stomata on the lower surface of a leaf.

Figure for question 7

(Source: © Rattiya Thongdumhyu/Shutterstock)

Figure 13

Measure the length of the line on the labelled guard cell in mm.

The actual length of the labelled guard cell is 0.05 mm.

Calculate the magnification of this image.

[3]
Reveal mark scheme Mark scheme for question 7(b)
(c)

Figure 14 shows the number of stomata per mm2 on the lower surface of leaves from plants growing in soils with different water content.

Figure for question 7

Explain the difference in the number of stomata per mm2 on the leaves of the plants grown in dry and wet soils.

[2]
Reveal mark scheme Mark scheme for question 7(c)
(d)

*(d) Figure 15a shows a plant that has been placed on its side.

Figure 15b shows the same plant after 5 days. maths symbol

Figure for question 7

Explain why the growth of the plant has changed.

[6]
Reveal mark scheme Mark scheme for question 7(d)

Paper 2 November 2021 Higher tier, Question 2 9 marks

Also involves Photosynthesis

Figure 2 shows an area of woodland with some small plants growing in the ground between the trees.

Figure for question 2

(Source: © Maksym Holovinov/Shutterstock)

Figure 2

(a)

Name a group of organisms that break down the dead leaves and release mineral ions into the soil.

[1]
Reveal mark scheme Mark scheme for question 2(a)
(b)

The mineral ions are absorbed from the soil into the roots of plants.

Describe how these mineral ions are transported from the roots to the leaves of the plants.

[2]
Reveal mark scheme Mark scheme for question 2(b)
(c)

A scientist recorded the mean light intensity and the mean number of small plants per m2 for six 25 m2 areas of the woodland.

Figure 3 shows the results.

Figure for question 2
(c)(i)

Explain the effect of light intensity on the number of small plants per m2.

[2]
Reveal mark scheme Mark scheme for question 2(c)(i)
(c)(ii)

State one variable the scientist should have controlled to make sure the light intensity measurements could be compared.

[1]
Reveal mark scheme Mark scheme for question 2(c)(ii)
(d)

The scientist selects an area near the edge of the woodland where many stinging nettles are growing.

This area is partly shaded by the trees.

Describe how the scientist should use a belt transect to investigate how light intensity affects the growth of stinging nettles.

[3]
Reveal mark scheme Mark scheme for question 2(d)

Paper 2 November 2020 Foundation tier, Question 8 12 marks

Figure 18 shows the leaves and flowers of water lily plants (Nymphaea odorata) on a lake.

Figure for question 8
(a)

Water lilies have stomata on the upper surface of the leaves.

Explain why water lilies have no stomata on the lower surface of the leaves.

[2]
Reveal mark scheme Mark scheme for question 8(a)
(b)(i)

The white petals of the water lily flowers cannot photosynthesise.

Which structure in leaf cells is the site of photosynthesis?

Question 8, shown as an image
[1]
Reveal mark scheme Mark scheme for question 8(b)(i)
(b)(ii)

Glucose is made by photosynthesis.

Glucose is converted to another sugar to be transported in the plant.

What is the name of this sugar?

Question 8, shown as an image
[1]
Reveal mark scheme Mark scheme for question 8(b)(ii)
(b)(iii)

Describe how this sugar is transported from the leaves to the flowers of the water lily.

[2]
Reveal mark scheme Mark scheme for question 8(b)(iii)
(c)

Figure 19 shows water lilies growing in a lake in Europe.

Figure for question 8
(c)(i)

One water lily plant was brought from America 10 years ago and planted in the lake shown in Figure 19.

Explain why this non‑indigenous plant now covers the whole surface of the lake.

[3]
Reveal mark scheme Mark scheme for question 8(c)(i)
(c)(ii)

Explain how the water lilies will affect the biodiversity of this lake.

[3]
Reveal mark scheme Mark scheme for question 8(c)(ii)

Paper 2 November 2020 Higher tier, Question 2 9 marks

Figure 2 shows the leaves and flowers of water lily plants (Nymphaea odorata) on a lake.

Figure for question 2
(a)

Water lilies have stomata on the upper surface of the leaves.

Explain why water lilies have no stomata on the lower surface of the leaves.

[2]
Reveal mark scheme Mark scheme for question 2(a)
(b)(i)

The white petals of the water lily flowers cannot photosynthesise.

Which structure in leaf cells is the site of photosynthesis?

Question 2, shown as an image
[1]
Reveal mark scheme Mark scheme for question 2(b)(i)
(b)(ii)

Glucose is made by photosynthesis.

Glucose is converted to another sugar to be transported in the plant.

What is the name of this sugar?

Question 2, shown as an image
[1]
Reveal mark scheme Mark scheme for question 2(b)(ii)
(b)(iii)

Describe how this sugar is transported from the leaves to the flowers of the water lily.

[2]
Reveal mark scheme Mark scheme for question 2(b)(iii)
(c)

Figure 3 shows water lilies growing in a lake in Europe.

Figure for question 2

One water lily plant was brought from America 10 years ago and planted in the lake shown in Figure 3.

Explain why this non‑indigenous plant now covers the whole surface of the lake.

[3]
Reveal mark scheme Mark scheme for question 2(c)

Paper 2 June 2019 Foundation tier, Question 3 7 marks

Also involves Plant hormones · Tropisms

(a) Photosynthesis occurs in leaves.

(a)
(a)(i)

Which substance is needed for photosynthesis?

Question 3, shown as an image
[1]
Reveal mark scheme Mark scheme for question 3(a)(i)
(a)(ii)

A leaf cell is 0.08 mm long.

Calculate the length of the image of this cell after it has been magnified 50 times using a microscope.

[2]
Reveal mark scheme Mark scheme for question 3(a)(ii)
(b)

Pine trees can live in dry soil.

Use words from the box to complete the sentences.

Figure for question 3

The pine leaf has stomata in pits to reduce the loss of........ .

The pine leaf is needle-shaped to reduce the surface........ .

[2]
Reveal mark scheme Mark scheme for question 3(b)
(c)

Figure 5 shows young tomato plants growing in a glasshouse.

Figure for question 3

The young tomato plants are growing towards the light.

Explain how a plant hormone causes these shoots to grow towards the light.

[2]
Reveal mark scheme Mark scheme for question 3(c)

Paper 2 June 2019 Higher tier, Question 1 7 marks

Also involves Gas exchange · Leaf structure · Adaptations

(a) Figure 1 shows a cross section of a leaf.

Figure for question 1
(a)
(a)(i)

What is the name of layer A?

Question 1, shown as an image
[1]
Reveal mark scheme Mark scheme for question 1(a)(i)
(a)(ii)

Explain the function of the spaces between the cells in layer B.

[2]
Reveal mark scheme Mark scheme for question 1(a)(ii)
(a)(iii)

Explain the function of part C in Figure 1.

[2]
Reveal mark scheme Mark scheme for question 1(a)(iii)
(b)

Xerophytes are plants adapted to live in very dry conditions.

State two differences between the leaf structure of a xerophyte and the leaf structure shown in Figure 1.

[2]
Reveal mark scheme Mark scheme for question 1(b)

Paper 2 June 2019 Higher tier, Question 5 9 marks

(a) (i) Water enters a plant through root hair cells.

Root hair cells have

Question 5, shown as an image
(a)(i)
[1]
Reveal mark scheme Mark scheme for question 5(a)(i)
(a)(ii)

Explain how water in the root is transported to the leaves of the plant.

[2]
Reveal mark scheme Mark scheme for question 5(a)(ii)
(b)

How is sucrose transported from the leaves to other parts of the plant?

Question 5, shown as an image
[1]
Reveal mark scheme Mark scheme for question 5(b)
(c)

Figure 7 shows the average size of stomata in a leaf during one day.

Figure for question 5
(c)(i)

Name the cells that change the size of stomata.

[1]
Reveal mark scheme Mark scheme for question 5(c)(i)
(c)(ii)

Describe the trend shown in Figure 7.

[2]
Reveal mark scheme Mark scheme for question 5(c)(ii)
(c)(iii)

The temperature increased from 8 am to 1 pm.

Explain why this affected the size of the stomata.

[2]
Reveal mark scheme Mark scheme for question 5(c)(iii)

Paper 2 June 2018 Foundation tier, Question 9 13 marks

Also involves Turgor and plant support

Figure for question 9
(a)(i)

State why a coverslip is placed on top of the leaf peel.

[1]
Reveal mark scheme Mark scheme for question 9(a)(i)
(a)(ii)

Explain why the leaf peel rather than the whole leaf was viewed with a microscope.

[2]
Reveal mark scheme Mark scheme for question 9(a)(ii)
(b)

The student drew a biological diagram of the leaf peel taken from the underside of the leaf.

Figure 23 shows this diagram.

Figure for question 9
(b)(i)

State the number of stomata visible on Figure 23.

[1]
Reveal mark scheme Mark scheme for question 9(b)(i)
(b)(ii)

The student observed that the stomata were open.

Describe how stomata open.

[3]
Reveal mark scheme Mark scheme for question 9(b)(ii)
(c)

*(c) Figure 24 shows xylem and phloem. Xylem and phloem are involved in the transport of substances through a plant.

Figure for question 9

Use Figure 24 to help you describe how water and sucrose move through a plant.

[6]
Reveal mark scheme Mark scheme for question 9(c)

Paper 2 June 2018 Higher tier, Question 3 9 marks

Also involves Leaf structure

A student compared the number of stomata on the upper and lower surfaces of a leaf.

She completed a leaf peel as shown in Figure 5.

Figure for question 3

leaf coated with clear layer of nail varnish layer of nail varnish placed nail varnish
removed on a microscope slide

Figure 5

The layer of nail varnish shows an impression of the cells on the surface of the leaf.

(a)(i)

State why a coverslip is placed on top of the leaf peel.

[1]
Reveal mark scheme Mark scheme for question 3(a)(i)
(a)(ii)

Explain why the leaf peel rather than the whole leaf was viewed with a microscope.

[2]
Reveal mark scheme Mark scheme for question 3(a)(ii)
(b)

The student drew a biological diagram of the leaf peel taken from the underside of the leaf.

Figure 6 shows this diagram.

Figure for question 3
(b)(i)

State the number of stomata visible on Figure 6.

[1]
Reveal mark scheme Mark scheme for question 3(b)(i)
(b)(ii)

The student observed that the stomata were open.

Describe how stomata open.

[3]
Reveal mark scheme Mark scheme for question 3(b)(ii)
(b)(iii)

The leaf peel from the upper surface of this leaf showed no stomata.

Explain why it is an advantage to the plant to have this distribution of stomata in the upper and lower surfaces of the leaf.

[2]
Reveal mark scheme Mark scheme for question 3(b)(iii)

Paper 2 June 2018 Higher tier, Question 8 11 marks

(a) Figure 16 shows the effect of light intensity and temperature on the rate of photosynthesis.

Figure for question 8
Question 8, shown as an image
(a)
(a)(i)

Describe the effect of light intensity on the rate of photosynthesis.

[2]
Reveal mark scheme Mark scheme for question 8(a)(i)
(a)(ii)

Explain the effect of temperature on the rate of photosynthesis.

[2]
Reveal mark scheme Mark scheme for question 8(a)(ii)
(b)

A student measured the rate of photosynthesis using algal balls in a laboratory. The tube of algal balls was kept at a temperature of 25 °C and was moved to different distances from a light source.

The results of this investigation showed that the rate of photosynthesis is

Question 8, shown as an image
[1]
Reveal mark scheme Mark scheme for question 8(b)
(c)

*(c) Explain how substances are moved through a plant by transpiration and translocation.

[6]
Reveal mark scheme Mark scheme for question 8(c)

Paper 2 June 2018 Higher tier, Question 10 11 marks

Also involves Plant hormones · Tropisms · Adaptations

(a) Figure 18 shows an investigation into the growth of plant shoots.

Experiment 1:

The tip of a shoot was removed from the plant.

There was no growth in the shoot after 3 days.

Figure for question 10

Experiment 2:

The tip of a shoot was cut off and then placed back onto the shoot.

The shoot had grown 6 mm after 3 days.

Figure for question 10
(a)
(a)(i)

Give one variable that must have been controlled for the plant shoot to grow vertically, as shown in experiment 2.

[1]
Reveal mark scheme Mark scheme for question 10(a)(i)
(a)(ii)

State one conclusion that could be made based on these two experiments.

[1]
Reveal mark scheme Mark scheme for question 10(a)(ii)
(a)(iii)

Which plant hormone causes growth in the plant shoot?

Question 10, shown as an image
[1]
Reveal mark scheme Mark scheme for question 10(a)(iii)
(a)(iv)

Explain one way that this investigation could be improved.

[2]
Reveal mark scheme Mark scheme for question 10(a)(iv)
(b)

*(b) Marram grass is a plant that grows on exposed areas of sand dunes.

Figure 19 shows marram grass growing and a cross section through a leaf of marram grass.

Figure for question 10

Explain how marram grass is adapted to survive in the hot, windy and dry conditions of a sand dune.

[6]
Reveal mark scheme Mark scheme for question 10(b)

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