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Edexcel GCSE Biology

Topic questions Cell biology Diffusion

Diffusion

14 questions · 146 marks · about 2.5 hours

Diffusion questions

Edexcel 1BI0 Diffusion 14 questions

Paper 1 June 2025 Foundation tier, Question 9 13 marks

Also involves Factors affecting enzyme activity Β· Surface area to volume ratio

(a)

Figure 17 shows a beaker containing a potato cylinder in distilled water.

Figure for question 9

Explain why the mass of this potato cylinder will change when it is placed in distilled water.

[3]
Reveal mark scheme Mark scheme for question 9(a)
(b)

A scientist investigated the rate of diffusion using three agar cubes of different sizes.

Each cube contained a pH indicator that changed from pink to colourless when placed in acid.

The cubes were placed in a beaker containing an acid.

Figure 18 shows some information about each cube.

Figure for question 9
(b)(i)

Calculate the volume of cube C.

[2]
Reveal mark scheme Mark scheme for question 9(b)(i)
(b)(ii)

Explain the difference in the times taken for cubes A and B to become colourless.

[2]
Reveal mark scheme Mark scheme for question 9(b)(ii)
(c)

* (c) Liquid agar was poured into a Petri dish and allowed to set.

A well was cut in the centre of the set agar.

Kiwi fruit juice was poured into the well.

Kiwi fruit juice contains an enzyme that causes set agar to break down into a liquid.

Figure 19 shows the results after 30 minutes.

Figure for question 9

Devise a plan to investigate the effect of different concentrations of kiwi fruit juice on the breakdown of set agar, using the equipment shown in Figure 19.

Your plan should include:

β€’ measurements that need to be taken

β€’ variables that need to be controlled.

[6]
Reveal mark scheme Mark scheme for question 9(c)

Paper 1 June 2025 Higher tier, Question 3 7 marks

Also involves Surface area to volume ratio

(a)

Figure 3 shows a beaker containing a potato cylinder in distilled water.

Figure for question 3

Explain why the mass of this potato cylinder will change when it is placed in distilled water.

[3]
Reveal mark scheme Mark scheme for question 3(a)
(b)

A scientist investigated the rate of diffusion using three agar cubes of different sizes.

Each cube contained a pH indicator that changed from pink to colourless when placed in acid.

The cubes were placed in a beaker containing an acid.

Figure 4 shows some information about each cube.

Figure for question 3
(b)(i)

Calculate the volume of cube C.

[2]
Reveal mark scheme Mark scheme for question 3(b)(i)
(b)(ii)

Explain the difference in the times taken for cubes A and B to become colourless.

[2]
Reveal mark scheme Mark scheme for question 3(b)(ii)

Paper 2 June 2025 Higher tier, Question 5 9 marks

(a)

A teacher was demonstrating a process to students.

The teacher had a beaker of water and added five drops of food colouring to the beaker.

Figure for question 5
(a)(i)

State the process being demonstrated.

[1]
Reveal mark scheme Mark scheme for question 5(a)(i)
(a)(ii)

A student asked if different food colourings spread evenly in the same amount of time.

Give two variables that would need to be controlled if the investigation was repeated with different food colourings.

[2]
Reveal mark scheme Mark scheme for question 5(a)(ii)
(b)

The teacher repeated this process with water at different temperatures. Figure 11 shows the results of this experiment.

Figure for question 5
(b)(i)

Draw a line graph to show the results in Figure 11.

[4]
Reveal mark scheme Mark scheme for question 5(b)(i)
(b)(ii)

Explain why the time taken decreased.

[2]
Reveal mark scheme Mark scheme for question 5(b)(ii)

Paper 2 June 2024 Foundation tier, Question 9 13 marks

(a)

Figure 13 shows a root hair cell from a plant.

Figure for question 9
(a)(i)

Name the part labelled X.

[1]
Reveal mark scheme Mark scheme for question 9(a)(i)
(a)(ii)

State one way that the structure of the root hair cell increases the volume of substances it absorbs.

[1]
Reveal mark scheme Mark scheme for question 9(a)(ii)
(a)(iii)

Explain why root hair cells do not contain chloroplasts.

[3]
Reveal mark scheme Mark scheme for question 9(a)(iii)
(b)
Question 9, shown as an image
Figure for question 9
Question 9, shown as an image
[2]
Reveal mark scheme Mark scheme for question 9(b)
(c)

*(c) The arrows in Figure 15 show the direction of water movement through a tree.

Figure for question 9

Explain how water is moved from the soil, through the plant and into the air.

[6]
Reveal mark scheme Mark scheme for question 9(c)

Paper 1 June 2022 Foundation tier, Question 4 10 marks

Also involves DNA and the genome

Alcohol is broken down by liver cells.

(a)

Which process moves alcohol from the blood into the liver cells?

Question 4, shown as an image
[1]
Reveal mark scheme Mark scheme for question 4(a)
(b)

If a person drinks too much alcohol, liver cells die and the person can develop cirrhosis of the liver.

The relative risk of developing cirrhosis of the liver is affected by two factors.

1. The volume of alcohol a person drinks in one week.

2. Whether the person drinks the alcohol on its own or with a meal.

Figure 7 shows how these two factors affect the relative risk of people developing cirrhosis of the liver.

Figure for question 4

Number of units of alcohol consumed per week

Figure 7

(b)(i)

Person A drinks alcohol on its own.

Person B drinks alcohol with their meals.

Calculate the difference in risk for these two people when each one drinks 28 units of alcohol per week.

[3]
Reveal mark scheme Mark scheme for question 4(b)(i)
(b)(ii)

Using evidence from Figure 7, state two pieces of health advice for people about drinking alcohol.

[2]
Reveal mark scheme Mark scheme for question 4(b)(ii)
(c)

Cystic fibrosis is a genetic condition that can also cause liver disease.

(c)(i)

State where genes are found in cells.

[1]
Reveal mark scheme Mark scheme for question 4(c)(i)
(c)(ii)

Figure 8 shows the inheritance of cystic fibrosis in a family.

F represents the dominant allele that does not cause cystic fibrosis.

f represents the recessive allele that causes cystic fibrosis.

Figure for question 4

person C person D
person E (ff)

A scientist states that the genotype of person B is Ff.

Explain why the scientist is correct.

[2]
Reveal mark scheme Mark scheme for question 4(c)(ii)
(c)(iii)

State the genotype of person C.

[1]
Reveal mark scheme Mark scheme for question 4(c)(iii)

Paper 2 November 2021 Foundation tier, Question 6 10 marks

Also involves Ventilation and the thorax Β· Response to exercise

(a)

Figure 10 shows a single-celled pond organism (Amoeba proteus).

Figure for question 6
(a)(i)

Which row of the table allows the net diffusion of oxygen into Amoeba proteus ?

Figure for question 6
[1]
Reveal mark scheme Mark scheme for question 6(a)(i)
(a)(ii)

Amoeba proteus uses oxygen for aerobic respiration.

Complete the equation for aerobic respiration.

glucose + oxygen maths symbol........ +........ (+ energy is released)

[1]
Reveal mark scheme Mark scheme for question 6(a)(ii)
(b)

Humans breathe faster when they exercise.

(b)(i)

Which two changes allow the rate of respiration in the muscle to increase?

Figure for question 6
[1]
Reveal mark scheme Mark scheme for question 6(b)(i)
(b)(ii)

Figure 11 shows breathing data for a human at rest and when running at 5 metres per second on a running machine.

Figure for question 6

Calculate the mean volume of air breathed per minute when running at 5 metres per second.

Give your answer to one decimal place.

........ dm3 per minute

[2]
Reveal mark scheme Mark scheme for question 6(b)(ii)
(b)(iii)

Devise an investigation to compare the mean number of breaths per minute for men, with the mean number of breaths per minute for women, when running at 5 metres per second on a running machine.

[4]
Reveal mark scheme Mark scheme for question 6(b)(iii)
(c)

Whilst running, the leg muscles of an athlete tightened up, causing cramp.

Name the product of anaerobic respiration that can cause cramp.

[1]
Reveal mark scheme Mark scheme for question 6(c)

Paper 2 November 2021 Foundation tier, Question 10 11 marks

Also involves The kidney and the urinary system

Figure 21 shows the human urinary system.

Figure for question 10
(a)

Urine is made by removing water, urea and salts from the blood.

Use the letter X to label where urine is made.

[1]
Reveal mark scheme Mark scheme for question 10(a)
(b)

Which substance is converted to urea in the liver?

Question 10, shown as an image
[1]
Reveal mark scheme Mark scheme for question 10(b)
(c)

A patient cannot remove enough urea from the blood when making urine.

Figure 22 shows a dialysis machine attached to the arm of this patient.

Their blood is pumped out of their arm, passed through the dialysis machine, and then put back into the patient.

Figure for question 10
(c)(i)

Describe what happens to urea as the blood passes through the dialysis machine.

[2]
Reveal mark scheme Mark scheme for question 10(c)(i)
(c)(ii)

Two patients need to have dialysis treatment.

Patient A needs this treatment three times a week.

Patient B needs this treatment once a week.

Give one reason why patient B needs dialysis treatment less often than patient A.

[1]
Reveal mark scheme Mark scheme for question 10(c)(ii)
(d)

Describe how to test for glucose in the dialysis fluid.

[3]
Reveal mark scheme Mark scheme for question 10(d)
(e)

Urease is an enzyme that breaks down urea into smaller molecules.

Explain why urease will not break down starch into smaller molecules.

[3]
Reveal mark scheme Mark scheme for question 10(e)

Paper 2 November 2021 Higher tier, Question 4 11 marks

Also involves The kidney and the urinary system Β· Excretion and the liver

Figure 7 shows the human urinary system.

Figure for question 4
(a)

Urine is made by removing water, urea and salts from the blood.

Use the letter X to label where urine is made.

[1]
Reveal mark scheme Mark scheme for question 4(a)
(b)

Which substance is converted to urea in the liver?

Question 4, shown as an image
[1]
Reveal mark scheme Mark scheme for question 4(b)
(c)

A patient cannot remove enough urea from the blood when making urine.

Figure 8 shows a dialysis machine attached to the arm of this patient.

Their blood is pumped out of their arm, passed through the dialysis machine, and then put back into the patient.

Figure for question 4
(c)(i)

Describe what happens to urea as the blood passes through the dialysis machine.

[2]
Reveal mark scheme Mark scheme for question 4(c)(i)
(c)(ii)

Two patients need to have dialysis treatment.

Patient A needs this treatment three times a week.

Patient B needs this treatment once a week.

Give one reason why patient B needs dialysis treatment less often than patient A.

[1]
Reveal mark scheme Mark scheme for question 4(c)(ii)
(d)

Describe how to test for glucose in the dialysis fluid.

[3]
Reveal mark scheme Mark scheme for question 4(d)
(e)

Urease is an enzyme that breaks down urea into smaller molecules.

Explain why urease will not break down starch into smaller molecules.

[3]
Reveal mark scheme Mark scheme for question 4(e)

Paper 2 November 2021 Higher tier, Question 5 9 marks

Figure 9 shows the structure and actual size of red blood cells (erythrocytes).

Figure for question 5

red blood cells side view of red blood cell (cut)

(Source: Β© N.Vinoth Narasingam/Shutterstock)

Figure 9

(a)(i)

Calculate the image size of the top view of this red blood cell if this cell is

Question 5, shown as an image

Give your answer in mm.

[2]
Reveal mark scheme Mark scheme for question 5(a)(i)
(a)(ii)

Explain how the shape of a red blood cell is related to its function.

[3]
Reveal mark scheme Mark scheme for question 5(a)(ii)
(a)(iii)

State the role of haemoglobin inside the red blood cell.

[1]
Reveal mark scheme Mark scheme for question 5(a)(iii)
(b)

Some blood transfusions contain red blood cells.

Red blood cells are stored in a solution containing 5.0% glucose and 0.9% salt.

Explain why red blood cells cannot be stored in pure water.

[3]
Reveal mark scheme Mark scheme for question 5(b)

Paper 1 November 2020 Foundation tier, Question 2 6 marks

(a)

Figure 4 shows three cells.

Figure for question 2
(a)(i)

What is structure X ?

Question 2, shown as an image
[1]
Reveal mark scheme Mark scheme for question 2(a)(i)
(a)(ii)

The bacterial cell in Figure 4 has a flagellum.

State the function of a flagellum.

[1]
Reveal mark scheme Mark scheme for question 2(a)(ii)
(a)(iii)

Give one other difference between the bacterial cell and the animal cell shown in Figure 4.

[1]
Reveal mark scheme Mark scheme for question 2(a)(iii)
(b)

Substances move into and out of cells.

How does oxygen move into and out of cells?

Question 2, shown as an image
[1]
Reveal mark scheme Mark scheme for question 2(b)
(c)

A plant leaf cell is 0.04 mm long.

Calculate the length of the image after this cell has been magnified 500 times.

[2]
Reveal mark scheme Mark scheme for question 2(c)

Paper 1 November 2020 Foundation tier, Question 4 10 marks

(a)

A student placed three different sized cubes of agar jelly into separate beakers containing the same concentration of hydrochloric acid.

The cubes contained a pink indicator.

This indicator becomes clear when in contact with an acid.

Figure 7 shows the results of the investigation after the cubes had been in the acid for 120 seconds.

Figure for question 4
(a)(i)

The distance from the outside of cube B to the pink area was 3 mm.

Calculate the distance diffused by hydrochloric acid in one second.

[2]
Reveal mark scheme Mark scheme for question 4(a)(i)
(a)(ii)

The student wanted to confirm their results.

Give one improvement the student should make to this investigation to confirm their results.

[1]
Reveal mark scheme Mark scheme for question 4(a)(ii)
(b)

Devise a method, using cubes of agar jelly, to investigate how temperature affects the rate of diffusion.

[3]
Reveal mark scheme Mark scheme for question 4(b)
(c)

Some substances move into and out of cells by active transport.

Which is the correct description of the movement of a substance by active transport?

Question 4, shown as an image
[1]
Reveal mark scheme Mark scheme for question 4(c)
(d)

Some drugs used to treat cancer are taken into cells by active transport.

Figure 8 shows some causes of preventable cases of cancer in 2015.

Figure for question 4
(d)(i)

What is the percentage of preventable cases of cancer that are caused by tobacco?

Question 4, shown as an image
[1]
Reveal mark scheme Mark scheme for question 4(d)(i)
(d)(ii)

In 2015, data from Cancer Research UK suggested that 163 440 cases of cancer could have been prevented.

Calculate the number of preventable cases of cancer caused by alcohol.

Give your answer to the nearest whole number.

number of preventable cases of cancer caused by alcohol........

[2]
Reveal mark scheme Mark scheme for question 4(d)(ii)

Paper 2 November 2020 Higher tier, Question 10 12 marks

Also involves The kidney and the urinary system Β· Ultrafiltration and selective reabsorption

(a)

A person with kidney disease may need kidney dialysis treatment.

Figure 15 shows an experiment to show how a dialysis membrane works.

Figure for question 10
Question 10, shown as an image
(a)(i)

Describe the method that would be used to find out what is present in the solution in the beaker, after one hour.

[4]
Reveal mark scheme Mark scheme for question 10(a)(i)
(a)(ii)

Explain how this experiment represents a simple model of kidney dialysis treatment.

[2]
Reveal mark scheme Mark scheme for question 10(a)(ii)
(b)

*(b) The nephrons in the kidney remove unwanted substances from the blood.

Figure 16 shows the structure of a nephron.

Figure for question 10

Explain how substances are exchanged between the blood entering the nephron and the filtrate leaving the collecting duct.

Include reference to named structures of the nephron in your answer.

[6]
Reveal mark scheme Mark scheme for question 10(b)

Paper 2 June 2019 Higher tier, Question 9 12 marks

(a) Figure 10 shows alveoli from a lung.

Figure for question 9

Figure 10

(a)
(a)(i)

Explain why these alveoli have the internal structure shown in Figure 10.

[3]
Reveal mark scheme Mark scheme for question 9(a)(i)
(a)(ii)

How does oxygen move across the alveolar membrane into the capillary?

Question 9, shown as an image
[1]
Reveal mark scheme Mark scheme for question 9(a)(ii)
(b)

Figure 11 shows the movement of molecules across a membrane.

Figure for question 9

at start
after 2 seconds
after 4 seconds

Figure 11

Describe how Figure 11 illustrates movement of molecules across a membrane.

[2]
Reveal mark scheme Mark scheme for question 9(b)
(c)

*(c) Explain, using Fick’s law, the factors that affect the diffusion rate of molecules into and out of cells.

[6]
Reveal mark scheme Mark scheme for question 9(c)

Paper 2 June 2018 Foundation tier, Question 7 13 marks

Also involves The kidney and the urinary system Β· Ultrafiltration and selective reabsorption Β· Excretion and the liver

Figure 16 shows the urinary system of a human.

Figure for question 7
(a)

Name the structures labelled P and Q.

[2]
Reveal mark scheme Mark scheme for question 7(a)
(b)

The kidney contains nephrons.

Figure 17 shows the concentration of glucose and protein found in the blood plasma and in the filtrate inside a nephron.

Figure for question 7
(b)(i)

Explain the difference in the concentration of protein in the blood plasma and in the filtrate in the nephron.

[2]
Reveal mark scheme Mark scheme for question 7(b)(i)
(b)(ii)

Explain how glucose moves from the blood plasma into the nephron.

[3]
Reveal mark scheme Mark scheme for question 7(b)(ii)
(c)

*(c) Figure 18 shows a patient undergoing kidney dialysis.

Figure for question 7

Describe how dialysis removes unwanted substances from the blood.

Include examples of unwanted substances in your answer.

[6]
Reveal mark scheme Mark scheme for question 7(c)

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